Mi Da, Li Zhen, Lim Lynette, Li Mingfeng, Moissidis Monika, Yang Yifei, Gao Tianliuyun, Hu Tim Xiaoming, Pratt Thomas, Price David J, Sestan Nenad, Marín Oscar
Centre for Developmental Neurobiology, Institute of Psychiatry, Psychology, and Neuroscience, King's College London, London SE1 1UL, UK.
Medical Research Council Centre for Neurodevelopmental Disorders, King's College London, London SE1 1UL, UK.
Science. 2018 Apr 6;360(6384):81-85. doi: 10.1126/science.aar6821. Epub 2018 Feb 22.
GABAergic interneurons (GABA, γ-aminobutyric acid) regulate neural-circuit activity in the mammalian cerebral cortex. These cortical interneurons are structurally and functionally diverse. Here, we use single-cell transcriptomics to study the origins of this diversity in the mouse. We identify distinct types of progenitor cells and newborn neurons in the ganglionic eminences, the embryonic proliferative regions that give rise to cortical interneurons. These embryonic precursors show temporally and spatially restricted transcriptional patterns that lead to different classes of interneurons in the adult cerebral cortex. Our findings suggest that shortly after the interneurons become postmitotic, their diversity is already patent in their diverse transcriptional programs, which subsequently guide further differentiation in the developing cortex.
γ-氨基丁酸能中间神经元（GABA，γ-氨基丁酸）调节哺乳动物大脑皮层的神经回路活动。这些皮层中间神经元在结构和功能上具有多样性。在这里，我们使用单细胞转录组学来研究小鼠中这种多样性的起源。我们在神经节隆起中鉴定出不同类型的祖细胞和新生神经元，神经节隆起是产生皮层中间神经元的胚胎增殖区域。这些胚胎前体细胞表现出时间和空间上受限的转录模式，这些模式导致成年大脑皮层中不同类别的中间神经元。我们的研究结果表明，中间神经元进入有丝分裂后期后不久，它们的多样性就已经在其不同的转录程序中显现出来，这些转录程序随后指导发育中的皮层进一步分化。
