Clinical Laboratory, Hospital Universitari de Bellvitge, L'Hospitalet de Llobregat, Barcelona, Spain.
Critical Care Unit, Hospital Universitari de Bellvitge, L'Hospitalet de Llobregat, Barcelona, Spain.
Biochem Med (Zagreb). 2018 Feb 15;28(1):010708. doi: 10.11613/BM.2018.010708.
The Sysmex XN-series haematology analyser has newly adopted a fluorescent channel to measure immature platelet fraction (IPF). To promote the clinical utility of this promising parameter, establishing a reliable reference interval is mandatory. According to previous studies, IPF values may be affected by the employed analyser and the ethnic background of the individual, but no differences seem to be found between individuals' genders. Therefore, this study aimed to define the reference interval for IPF in a Spanish population following Clinical and Laboratory Standard Institute (CLSI) guidelines.
A total of 153 healthy Caucasian adults from Spain met the inclusion criteria. IPF measurement was performed by means of a Sysmex XN-2000 haematology analyser. A non-parametric percentile method was used to calculate the reference intervals in accordance with CLSI guidelines.
The obtained reference interval for IPF on the Sysmex XN-2000 was 1.6-9.6% (90% confidence intervals (CIs) were 1.5-1.8 and 9.3-11.5, respectively). No significant gender difference in IPF reference intervals was observed (P = 0.101).
This study provides, for the first time, a reference interval for IPF using a Sysmex XN-2000 in a Spanish population, ranging from 1.6 to 9.6%. These data are needed to evaluate platelet production in several conditions such as thrombocytopenia, inflammatory states and cardiovascular diseases, as well as for future research.
希森美康 XN 系列血液分析仪采用新的荧光通道来测量未成熟血小板分数（IPF）。为了促进这一有前途的参数的临床应用，建立一个可靠的参考区间是必要的。根据之前的研究，IPF 值可能会受到所使用的分析仪和个体的种族背景的影响，但个体的性别之间似乎没有差异。因此，本研究旨在根据临床和实验室标准协会（CLSI）的指南，为西班牙人群定义 IPF 的参考区间。
共有 153 名符合条件的西班牙白种成年人纳入本研究。采用希森美康 XN-2000 血液分析仪测量 IPF。根据 CLSI 指南，使用非参数百分位数法计算参考区间。
在希森美康 XN-2000 上获得的 IPF 参考区间为 1.6-9.6%（90%置信区间（CI）分别为 1.5-1.8 和 9.3-11.5）。IPF 参考区间在性别之间无显著差异（P = 0.101）。
本研究首次在西班牙人群中使用希森美康 XN-2000 提供了 IPF 的参考区间，范围为 1.6-9.6%。这些数据对于评估血小板生成在几种情况下是必要的，如血小板减少症、炎症状态和心血管疾病，以及未来的研究。
