Ali Usman, Knight Gavin, Gibbs Roz, Tsitsikas Dimitris A
a Department of Haematology , Homerton University Hospital NHS Foundation Trust , London , UK.
b School of Pharmacy and Biomedical Sciences , University of Portsmouth , Portsmouth , UK.
Scand J Clin Lab Invest. 2017 Dec;77(8):658-664. doi: 10.1080/00365513.2017.1394488. Epub 2017 Nov 9.
Immature platelet fraction (IPF) estimation is a non-invasive and sensitive test that is available on recently introduced Sysmex XN-series of automated haematology analysers. It is a direct cellular indicator of thrombopoiesis. The aim of this study was to establish reference intervals for IPF, for both absolute (A-IPF) and percentage (%-IPF) measurements.
A total of 2366 samples that met the inclusion criteria were assayed for full blood count on the Sysmex XN-10 and a non-parametric percentile method was used for calculating the reference intervals.
After the outliers were excluded, the reference interval for %-IPF and A-IPF on Sysmex XN-10 were 1.6-10.1% and 4.37-23.21 × 10/L in total individuals, respectively. There was a statistical significance noted between the sexes (p = .004) for %-IPF, therefore a sex-specific reference interval was established, which was 1.8-10.0% for the males and 1.5-10.1% for females. No significant difference in sex status for A-IPF and age status for both %-IPF and A-IPF was observed. A very poor correlation was estimated between age versus %-IPF, ρ = 0.0156, and age versus A-IPF, ρ = -0.0023, indicating that there is no overall biological relationship between age and these parameters. As expected, a strong correlation between %-IPF and A-IPF was noted which could be attributed to their inter-relatedness.
This large-scale study showed comparable reference intervals with the previous studies for %-IPF and A-IPF in a UK population. It found the need to establish sex-specific reference intervals for %-IPF, but not for A-IPF, whereas reference intervals were found to be stable across the age range.
未成熟血小板分数（IPF）测定是一种非侵入性且灵敏的检测方法，可通过最近推出的Sysmex XN系列自动血液分析仪进行检测。它是血小板生成的直接细胞指标。本研究的目的是建立IPF绝对值（A-IPF）和百分比（%-IPF）测量的参考区间。
共2366份符合纳入标准的样本在Sysmex XN-10上进行全血细胞计数检测，并采用非参数百分位数法计算参考区间。
排除异常值后，Sysmex XN-10上%-IPF和A-IPF在所有个体中的参考区间分别为1.6 - 10.1%和4.37 - 23.21×10⁹/L。%-IPF在性别之间存在统计学差异（p = 0.004），因此建立了性别特异性参考区间，男性为1.8 - 10.0%，女性为1.5 - 10.1%。未观察到A-IPF的性别差异以及%-IPF和A-IPF的年龄差异。年龄与%-IPF之间的相关性很差，ρ = 0.0156，年龄与A-IPF之间的相关性也很差，ρ = -0.0023，表明年龄与这些参数之间不存在总体生物学关系。正如预期的那样，%-IPF和A-IPF之间存在很强的相关性，这可归因于它们的相互关联性。
这项大规模研究表明，在英国人群中，%-IPF和A-IPF的参考区间与先前的研究结果相当。研究发现有必要为%-IPF建立性别特异性参考区间，但A-IPF无需建立，而参考区间在整个年龄范围内是稳定的。
