Fei Peng, Jiang Yichao, Gong Shaoying, Li Ran, Jiang Yan, Yuan Xiujuan, Wang Ziyuan, Kang Huaibin, Ali Md Aslam
College of Food and Biological Engineering, Henan University of Science and Technology, Luoyang 471023, People's Republic of China.
Changbai Mountains Food and Drug Inspection Testing Center, Baishan 134511, People's Republic of China.
J Food Prot. 2018 Mar 1;81(3):456-460. doi: 10.4315/0362-028X.JFP-17-326.
Cronobacter species (formerly Enterobacter sakazakii) are emerging opportunistic bacterial pathogens that can infect both infants and adults. This study was conducted to isolate and genotype diverse Cronobacter species from drinking water, chilled fresh pork, powdered infant formula, instant noodles, cookies, fruits, vegetables, and dishes in Northeast China and to evaluate the antibiotic resistance and susceptibility of the isolates. Thirty-four Cronobacter strains were isolated and identified: 21 C. sakazakii isolates (61.8%), 10 C. malonaticus isolates (29.4%), 2 C. dublinensis isolates (5.9%), and 1 C. turicensis isolate (2.9%). These isolates were further divided into 15 sequence types (STs) by multilocus sequence typing. C. sakazakii ST4 (10 isolates, 29.4%), ST1 (3 isolates, 8.8%), and ST8 (3 isolates, 8.8%) and C. malonaticus ST7 (four isolates, 11.8%) were dominant. Antibiotic susceptibility testing indicated that all 34 Cronobacter isolates were susceptible to ampicillin-sulbactam, cefotaxime, ciprofloxacin, gentamicin, meropenem, tetracycline, piperacillin-tazobactam, and trimethoprim-sulfamethoxazole, 88.2% were susceptible to chloramphenicol, and 67.6% were resistant to cephalothin. The results of this study enhance knowledge about genotyping and antibiotic resistance of these Cronobacter species and could be used to prevent potential hazards caused by these strains in drinking water and various food products.
阪崎肠杆菌属（以前称为阪崎肠杆菌）是新兴的机会性细菌病原体，可感染婴儿和成人。本研究旨在从中国东北地区的饮用水、冷藏鲜猪肉、婴儿配方奶粉、方便面、饼干、水果、蔬菜和菜肴中分离不同的阪崎肠杆菌属并进行基因分型，并评估分离株的抗生素耐药性和敏感性。共分离鉴定出34株阪崎肠杆菌属菌株：21株阪崎肠杆菌分离株（61.8%）、10株丙二酸阪崎肠杆菌分离株（29.4%）、2株都柏林阪崎肠杆菌分离株（5.9%）和1株苏黎世阪崎肠杆菌分离株（2.9%）。通过多位点序列分型将这些分离株进一步分为15个序列型（STs）。阪崎肠杆菌ST4（10株，29.4%）、ST1（3株，8.8%）和ST8（3株，8.8%）以及丙二酸阪崎肠杆菌ST7（4株，11.8%）占主导地位。抗生素敏感性测试表明，所有34株阪崎肠杆菌属分离株对氨苄西林-舒巴坦、头孢噻肟、环丙沙星、庆大霉素、美罗培南、四环素、哌拉西林-他唑巴坦和甲氧苄啶-磺胺甲恶唑敏感，88.2%对氯霉素敏感，67.6%对头孢菌素耐药。本研究结果增进了对这些阪崎肠杆菌属基因分型和抗生素耐药性的了解，可用于预防这些菌株在饮用水和各种食品中造成的潜在危害。
