Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangdong, Academy of Sciences, Guangzhou 510070, China.
Department of Food Science &Technology, Jinan University, Guangzhou, China.
Int J Food Microbiol. 2020 Dec 16;335:108857. doi: 10.1016/j.ijfoodmicro.2020.108857. Epub 2020 Sep 7.
Cronobacter species (Cronobacter spp.) are important foodborne pathogens that can infect and cause serious life-threatening diseases in infants and immunocompromised elderly. This study aimed to acquire data on Cronobacter spp. contamination of aquatic products in China from 2011 to 2016. In total, 800 aquatic products were tested, and the overall contamination rate for Cronobacter spp. was 3.9% (31/800). The average contamination level of the positive samples was 2.05 MPN/g. Four species and nine serotypes were identified among 33 isolates, of which the C. sakazakii serogroup O1 (n = 9) was the primary serotype. The majority of Cronobacter spp. strains harbored highest resistance against cephalothin (84.8%), followed by tetracycline (6.1%), trimethoprim/sulfameth-oxazole (3.0%) and chloramphenicol (3.0%). Two isolates were resistant to three antibiotics. In total, 26 sequence types and 33 CRISPR types (including 6 new STs and 26 new CTs) were identified, which indicates the extremely high diversity of Cronobacter spp. in aquatic products. Pathogenic C. sakazakii ST4, ST1, and C. malonaticus ST7 were also observed. Overall, this large-scale study revealed the relatively low prevalence and high genetic diversity of Cronobacter spp. in aquatic products in China, and the findings provide valuable information that can guide the establishment of effective measures for the control and precaution of Cronobacter spp. in aquatic products during production processes.
克罗诺杆菌属（Cronobacter spp.）是重要的食源性致病菌，可感染并导致婴儿和免疫功能低下的老年人发生严重的危及生命的疾病。本研究旨在获取 2011 年至 2016 年中国水产食品中克罗诺杆菌属污染的数据。共检测了 800 种水产食品，克罗诺杆菌属污染的总体检出率为 3.9%（31/800）。阳性样品的平均污染水平为 2.05 MPN/g。在 33 株分离株中鉴定出 4 个种和 9 个血清型，其中 C. sakazakii 血清群 O1（n=9）是主要血清型。大多数克罗诺杆菌属菌株对头孢噻吩的耐药性最高（84.8%），其次是四环素（6.1%）、甲氧苄啶/磺胺甲恶唑（3.0%）和氯霉素（3.0%）。有 2 株分离株对 3 种抗生素耐药。共鉴定出 26 种序列类型和 33 种 CRISPR 类型（包括 6 种新 ST 型和 26 种新 CT 型），表明水产食品中克罗诺杆菌属的多样性极高。还观察到致病性 C. sakazakii ST4、ST1 和 C. malonaticus ST7。总体而言，这项大规模研究揭示了中国水产食品中克罗诺杆菌属的流行率相对较低，遗传多样性较高，研究结果为生产过程中控制和预防水产食品中克罗诺杆菌属提供了有价值的信息。
