Holmes Hal R, Vlaisavljevich Eli, Tan Ee Lim, Snyder Katherine L, Ong Keat Ghee, Rajachar Rupak M
Michigan Technological University, Department of Biomedical Engineering, Houghton, MI 49931, USA.
Michigan Technological University, Department of Biomedical Engineering, Houghton, MI 49931, USA.
J Biomech. 2018 Apr 11;71:199-207. doi: 10.1016/j.jbiomech.2018.02.007. Epub 2018 Feb 11.
The effect of sub-cellular mechanical loads on the behavior of fibroblasts was investigated using magnetoelastic (ME) materials, a type of material that produces mechanical vibrations when exposed to an external magnetic AC field. The integration of this functionality into implant surfaces could mitigate excessive fibrotic responses to many biomedical devices. By changing the profiles of the AC magnetic field, the amplitude, duration, and period of the applied vibrations was altered to understand the effect of each parameter on cell behavior. Results indicate fibroblast adhesion depends on the magnitude and total number of applied vibrations, and reductions in proliferative activity, cell spreading, and the expression of myofibroblastic markers occur in response to the vibrations induced by the ME materials. These findings suggest that the subcellular amplitude mechanical loads produced by ME materials could potentially remotely modulate myofibroblastic activity and limit undesirable fibrotic development.
利用磁弹性（ME）材料研究了亚细胞机械负荷对成纤维细胞行为的影响，磁弹性材料是一种在暴露于外部交流磁场时会产生机械振动的材料。将此功能集成到植入物表面可以减轻对许多生物医学设备的过度纤维化反应。通过改变交流磁场的分布，改变施加振动的幅度、持续时间和周期，以了解每个参数对细胞行为的影响。结果表明，成纤维细胞的粘附取决于施加振动的幅度和总数，并且响应于ME材料诱导的振动，增殖活性、细胞铺展和肌成纤维细胞标志物的表达会降低。这些发现表明，ME材料产生的亚细胞幅度机械负荷可能潜在地远程调节肌成纤维细胞活性并限制不良纤维化发展。
