Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA.
IEEE Trans Biomed Eng. 2011 Mar;58(3):698-704. doi: 10.1109/TBME.2010.2093131. Epub 2010 Nov 18.
Interfacial fibrosis is known to dramatically decrease the lifespan, stability, and function of biomedical implants and bone-anchored prosthetics. Bioactive coatings aimed at mitigating fibrous adhesions are one of the approaches to alleviate the problem. In this paper, we are developing a bioactive coating based upon a magnetoelastic (ME) material that vibrates in response to an ac magnetic field. In order to establish these coatings for this purpose, the ME material was first rendered bioactive through the sequential addition of polyurethane and chitosan thin films. Indirect live/dead assays were performed showing increased cell viability for polyurethane and chitosan-coated sensors compared to the uncoated controls. Direct adhesion experiments were performed to test the response of fibroblasts cultured on static and vibrated ME materials. Results showed cells adherent to static but not vibrated coatings. Detached cells showed no viability loss compared to controls. The finding that submicrometer ME vibrations can prevent cell adhesion in vitro without inducing cell death suggests the potential of these coatings to effectively control interfacial fibrosis. Future work will address the effect of vibrations on cell morphology and local gene expression in vitro, as well as fibrous tissue formation in vivo.
界面纤维化会极大地缩短生物医学植入物和骨锚假体的寿命、稳定性和功能。旨在减轻纤维粘连的生物活性涂层是缓解该问题的方法之一。在本文中，我们正在开发一种基于磁弹（ME）材料的生物活性涂层，该材料会在交流磁场的作用下振动。为了将这种涂层应用于此，首先通过顺序添加聚氨酯和壳聚糖薄膜使 ME 材料具有生物活性。间接死活检测表明，与未涂层的对照相比，涂有聚氨酯和壳聚糖的传感器的细胞活力增加。进行直接粘附实验以测试在静态和振动 ME 材料上培养的成纤维细胞的反应。结果表明，细胞在静态但不在振动涂层上附着。与对照相比，分离的细胞没有活力损失。亚微米级 ME 振动可以防止体外细胞粘附而不会诱导细胞死亡的发现表明，这些涂层有可能有效地控制界面纤维化。未来的工作将解决振动对体外细胞形态和局部基因表达以及体内纤维组织形成的影响。
