通过应用统计方法优化NRC1731介导的银纳米颗粒生物合成：增强与表征
Optimization of silver nanoparticles biosynthesis mediated by NRC1731 through application of statistical methods: enhancement and characterization.
作者信息
Elsayed Maysa A, Othman Abdelmageed M, Hassan Mohamed M, Elshafei Ali M
机构信息
Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre (NRC), 33 El Bohouth St., Dokki, Giza, 12622 Egypt.
出版信息
3 Biotech. 2018 Mar;8(3):132. doi: 10.1007/s13205-018-1158-6. Epub 2018 Feb 15.
Abstract
The fungal-mediated silver nanoparticles (AgNPs) biosynthesis optimization via the application of central composite design (CCD) response surface to develop an effective ecofriendly and inexpensive green process was the aim of the current study. Nanosilver biosynthesis using the NRC1731 cell-free filtrate (CFF) was studied through involving the most parameters affecting the AgNPs green synthesis and its interactions effects. The statistical optimization models showed that using 59.37% of CFF in reaction containing 1.82 mM silver nitrate for 34 h at pH 7.0 is the optimum value to optimize the AgNPs biosynthesis. The obtained AgNPs were characterized by means of electron microscopy, UV/visible spectrophotometry, energy dispersive X-ray analysis and infrared spectroscopy to elucidate its almost spherical shape with diameter of 3-20 nm. The produced AgNPs exhibited a considerable antimicrobial activity against , in addition to .
摘要
本研究旨在通过应用中心复合设计（CCD）响应面法优化真菌介导的银纳米颗粒（AgNPs）生物合成，以开发一种有效、环保且廉价的绿色工艺。通过纳入影响AgNPs绿色合成的最主要参数及其相互作用效应，研究了利用NRC1731无细胞滤液（CFF）进行纳米银生物合成的过程。统计优化模型表明，在pH值为7.0、含有1.82 mM硝酸银的反应中使用59.37%的CFF并反应34小时是优化AgNPs生物合成的最佳值。通过电子显微镜、紫外/可见分光光度法、能量色散X射线分析和红外光谱对所得AgNPs进行表征，以阐明其几乎呈球形，直径为3 - 20 nm。除了……之外，所制备的AgNPs对……还表现出相当强的抗菌活性。
需注意，原文中“against, in addition to.”部分内容不完整，翻译时保留了原文格式。
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