真菌蛋白诱导合成银纳米颗粒及其表征与在不同生物活性中的应用
Biosynthesis and characterization of silver nanoparticles induced by fungal proteins and its application in different biological activities.
作者信息
Othman Abdelmageed M, Elsayed Maysa A, Al-Balakocy Naser G, Hassan Mohamed M, Elshafei Ali M
机构信息
Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt.
Protein and Manmade Fibers Department, Textile Research Division, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt.
出版信息
J Genet Eng Biotechnol. 2019 Nov 1;17(1):8. doi: 10.1186/s43141-019-0008-1.
BACKGROUND
The present study aims to apply an efficient eco-friendly and inexpensive process for green synthesis of silver nanoparticles (AgNPs) through the mediation of fungal proteins from Aspergillus fumigatus DSM819, characterization, and its application as antimicrobial finishing agent in textile fabrics against some infectious microorganisms.
RESULTS
Optimum conditions for AgNP biosynthesis could be achieved by means of using 60% (v/v) of cell-free filtrate (CFF) and 1.5 mM of AgNO at pH 10.0 after 90 min. The obtained AgNPs were of spherical shape with 90% of distribution below than 84.4 nm. The biosynthesized AgNPs exerted an antimicrobial activity against the studied pathogenic microorganisms (E. coli, B. mycoides, and C. albicans). In addition, IC values against in vitro tumor cell lines were found to be 31.1, 45.4, 40.9, and 33.5 μg/ml for HCT116, A549, MCF7, and PC3, respectively. Even with a very low concentration (0.25%), the treated PET/C fabrics by AgNPs exerted an antimicrobial activity against E. coli, B. mycoides, and C. albicans to give inhibition zone diameter of 15, 15, and 16 mm, respectively.
CONCLUSIONS
The green biosynthesis approach applied in this study is a non-toxic alternative to the traditional chemical and physical methods, and would be appropriate for biological large-scale production and prospective treatments.
背景
本研究旨在通过烟曲霉DSM819的真菌蛋白介导，应用一种高效、环保且廉价的方法来绿色合成银纳米颗粒（AgNPs），对其进行表征，并将其作为抗菌整理剂应用于纺织面料，以对抗某些传染性微生物。
结果
通过在pH值为10.0的条件下，使用60%（v/v）的无细胞滤液（CFF）和1.5 mM的AgNO，90分钟后可实现AgNP生物合成的最佳条件。所获得的AgNPs呈球形，90%的粒径分布低于84.4 nm。生物合成的AgNPs对所研究的致病微生物（大肠杆菌、蕈状芽孢杆菌和白色念珠菌）具有抗菌活性。此外，发现对体外肿瘤细胞系的IC值分别为：HCT116为31.1 μg/ml、A549为45.4 μg/ml、MCF7为40.9 μg/ml、PC3为33.5 μg/ml。即使浓度非常低（0.25%），经AgNPs处理的PET/C织物对大肠杆菌、蕈状芽孢杆菌和白色念珠菌仍具有抗菌活性，抑菌圈直径分别为15、15和16 mm。
结论
本研究中应用的绿色生物合成方法是传统化学和物理方法的无毒替代方法，适用于生物大规模生产和前瞻性治疗。
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