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利用鹿角菜( Gelidium amansii )合成并表征银纳米粒子及其对各种病原菌的抗菌性能。

Synthesis and characterization of silver nanoparticles using Gelidium amansii and its antimicrobial property against various pathogenic bacteria.

机构信息

Green Processing, Bioremediation and Alternative Energies Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam.

Anna University, ACT Campus, Chennai, Tamil Nadu 25, India.

出版信息

Microb Pathog. 2018 Jan;114:41-45. doi: 10.1016/j.micpath.2017.11.013. Epub 2017 Nov 14.

Abstract

Microfouling is evolving at a fast rate causing augmented mortality rates and damage worldwide. Until now, several remedial measures have been exploited to overcome microfouling, amongst them nanoparticles play a superior role. Currently, green synthesized nanoparticles have been centered owing to its eco-friendly, cost effectively and non-toxic nature which has also increased its industrial applications (biomedicine, food and textile). In the present research Silver Nanoparticles (Ag NPs) synthesized using marine red algae Gelidium amansii. The synthesized Ag NPs were characterized using UV-Vis Spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Further the antibacterial potentials of Ag NPs were evaluated against pathogenic Gram positive (Staphylococcus aureus, Bacillus pumilus) and Gram negative bacterial (Escherichia coli, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Aeromonas hydrophila) pathogens. Our findings suggest that Ag NPs synthesized using a green approach effectively reduce the bacterial growth by eliciting a bactericidal activity against the Gram Negative and Gram Positive biofilm forming pathogens. Thereby, Ag NPs synthesized using G. amansii could reflect as potential anti micro-fouling coatings for various biomedical and environmental applications.

摘要

微生物污染正在迅速发展,导致全球死亡率和损失增加。到目前为止,已经开发了几种补救措施来克服微生物污染,其中纳米颗粒起着重要作用。目前,由于其环保、经济高效和无毒的特性,绿色合成的纳米颗粒已成为研究重点,这也增加了其在工业中的应用(生物医学、食品和纺织)。本研究采用海洋红藻 Gelidium amansii 合成了银纳米颗粒(Ag NPs)。采用紫外-可见分光光度法、傅里叶变换红外光谱(FTIR)和扫描电子显微镜(SEM)对合成的 Ag NPs 进行了表征。进一步评估了 Ag NPs 对致病性革兰氏阳性(金黄色葡萄球菌、短小芽孢杆菌)和革兰氏阴性细菌(大肠杆菌、铜绿假单胞菌、副溶血弧菌、嗜水气单胞菌)的抗菌潜力。我们的研究结果表明,使用绿色方法合成的 Ag NPs 通过对革兰氏阴性和革兰氏阳性生物膜形成病原体产生杀菌活性,有效地抑制了细菌的生长。因此,使用 G. amansii 合成的 Ag NPs 可作为各种生物医学和环境应用的潜在抗微生物污染涂层。

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