College of Agriculture, Ningxia University, Yinchuan, China.
College of Agriculture, Ningxia University, Yinchuan, China.
Microb Pathog. 2018 Apr;117:310-314. doi: 10.1016/j.micpath.2018.02.042. Epub 2018 Feb 23.
Florfenicol is an antibiotic, a fluorinated structural analogue of thiamphenicol and chloramphenicol, approved exclusively for use in Asia for aquaculture since the 1980's. Our study examined the prevalence of florfenicol resistance in bovine mastitis Escherichia coli isolates. A total of 245 E. coli isolates were collected from bovine mastitis in Ningxia Province, China between May 2016 to July 2017 and screened for florfenicol resistance gene, floR gene by PCR analysis. About 7.35% (15/245) of the isolates were positive for floR gene. Minimal inhibitory concentration (MIC) results showed that 9 isolates were susceptible strains and 6 isolates were highly resistant to florfenicol. HPLC (high performance liquid chromatography) results showed that the amounts of florfenicol was significantly improved in the presence of carbonyl cyanide 3-chlorophenylhydrazone (CCCP) as an efflux pump inhibitor. This, therefore, indicates that the employment of florfenicol in conjunction with CCCP in future drug formulations should be considered.
氟苯尼考是一种抗生素，是甲砜霉素和氯霉素的氟化结构类似物，自 20 世纪 80 年代以来，仅在亚洲被批准用于水产养殖。我们的研究检测了牛乳腺炎大肠杆菌分离株中氟苯尼考耐药的流行情况。2016 年 5 月至 2017 年 7 月期间，我们从中国宁夏的牛乳腺炎中采集了 245 株大肠杆菌分离株，并通过 PCR 分析筛选了氟苯尼考耐药基因 floR 基因。大约 7.35%（15/245）的分离株 floR 基因呈阳性。最小抑菌浓度（MIC）结果显示，有 9 株分离株对氟苯尼考敏感，6 株分离株对氟苯尼考高度耐药。高效液相色谱（HPLC）结果表明，在羰基氰化物 3-氯苯腙（CCCP）作为外排泵抑制剂存在的情况下，氟苯尼考的量显著增加。因此，这表明在未来的药物配方中应考虑将氟苯尼考与 CCCP 联合使用。
