Wu Xiangyun, Liu Jiayi, Feng Jiawei, Shabbir Muhammad Abu Bakr, Feng Yali, Guo Rui, Zhou Meifang, Hou Sulin, Wang Guiqiang, Hao Haihong, Cheng Guyue, Wang Yulian
MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, China.
Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Front Microbiol. 2022 May 4;13:858799. doi: 10.3389/fmicb.2022.858799. eCollection 2022.
() is an opportunistic pathogen, which causes serious infections in humans and animals. To investigate the antimicrobial resistance pattern and virulence profile of , a total of 887 samples were collected from both the healthy and mastitis cows and the bedding, feed, feces, air, drinking water, spraying water, washing water, and milk cup swabs from five dairy farms in Hubei, China, during 2019 and 2020. was isolated and identified using PCR of the and 16S rDNA sequencing. A genotypic characterization was performed for isolates using typing multilocus sequence typing (MLST). Antimicrobial resistances were confirmed using broth microdilution against 17 antimicrobial agents and resistance and virulence genes were determined by PCR. The prevalence of was 26.94% (239/887) distributed in 101 allele types (199/239, 83.26%) and 100 sequence types (STs) (209/239, 87.45%), including 5 new allele type and 25 new STs. Phylogenetic analysis showed that isolated from milk, nipple swab, feed, and feces is classified in the same clone complex. By comparing with the PubMLST database, at least 67 STs have the risk of spreading in different species and regions. Interestingly, 60 STs have been isolated from humans. The isolates were highly sensitive to meropenem and colistin, but resistant to ampicillin (100%), sulfisoxazole (94.56%), cephalothin (47.28%), streptomycin (30.13%), and so on. Noteworthy, multidrug-resistant (MDR) rate was found to be 43.93% in this study. By PCR, 30 of 68 antimicrobial resistance (AMR) genes were identified; the prevalence rate of , , , , , and was more than 50%. Eleven -producing were found. The detection rate of , , , , , , , and was over 85%. This study reinforces the epidemiological importance of in food-producing animals in Hubei. The emergence and spread of environmental MDR may pose a potential threat to food safety and public health.
（）是一种机会致病菌，可在人类和动物中引起严重感染。为调查（）的抗菌药物耐药模式和毒力特征，于2019年至2020年期间从中国湖北五个奶牛场的健康奶牛和患乳腺炎奶牛以及垫料、饲料、粪便、空气、饮用水、喷淋水、冲洗水和奶杯拭子中总共采集了887份样本。通过（）基因的PCR和16S rDNA测序对（）进行分离和鉴定。使用（）分型和多位点序列分型（MLST）对（）分离株进行基因分型。采用肉汤微量稀释法针对17种抗菌药物确认抗菌药物耐药性，并通过PCR测定耐药基因和毒力基因。（）的流行率为26.94%（239/887），分布于101种（）等位基因类型（199/239，83.26%）和100种序列类型（STs）（209/239，87.45%），包括5种新的（）等位基因类型和25种新的STs。系统发育分析表明，从牛奶、乳头拭子、饲料和粪便中分离出的（）属于同一克隆复合体。与PubMLST数据库比较发现，至少67种STs有在不同物种和地区传播的风险。有趣的是，已从人类中分离出60种STs。分离株对美罗培南和黏菌素高度敏感，但对氨苄西林（100%）、磺胺异恶唑（94.56%）、头孢噻吩（47.28%）、链霉素（30.13%）等耐药。值得注意的是，本研究中发现多重耐药（MDR）率为43.93%。通过PCR，在68种抗菌药物耐药（AMR）基因中鉴定出30种；（）、（）、（）、（）、（）和（）的流行率超过50%。发现11株产（）的（）。（）、（）、（）、（）、（）、（）、（）和（）的检出率超过85%。本研究强化了（）在湖北产食动物中的流行病学重要性。环境中多重耐药（）的出现和传播可能对食品安全和公共卫生构成潜在威胁。
