Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM , Nicolás Cabrera, 9 Campus de Cantoblanco, CEI UAM+CSIC , 28049 Madrid , Spain.
IMDEA Alimentación , Carretera de Canto Blanco no. 8 , 28049 Madrid , Spain.
J Proteome Res. 2018 Apr 6;17(4):1624-1635. doi: 10.1021/acs.jproteome.7b00904. Epub 2018 Mar 9.
Moderate red-wine consumption has been widely described to exert several benefits in human health. This is mainly due to its unique content of bioactive polyphenols, which suffer several modifications along their pass through the digestive system, including microbial transformation in the colon and phase-II metabolism, until they are finally excreted in urine and feces. To determine the impact of moderate wine consumption in the overall urinary metabolome of healthy volunteers ( n = 41), samples from a red-wine interventional study (250 mL/day, 28 days) were investigated. Urine (24 h) was collected before and after intervention and analyzed by an untargeted ultrahigh-performance liquid chromatography-time-of-flight mass spectrometry metabolomics approach. 94 compounds linked to wine consumption, including specific wine components (tartaric acid), microbial-derived phenolic metabolites (5-(dihydroxyphenyl)-γ-valerolactones and 4-hydroxyl-5-(phenyl)-valeric acids), and endogenous compounds were identified. Also, some relationships between parallel fecal and urinary metabolomes are discussed.
适量饮酒被广泛认为对人体健康有多种益处。这主要是由于其独特的生物活性多酚含量，这些多酚在通过消化系统的过程中会发生多种变化，包括在结肠中的微生物转化和 II 相代谢，直到最终在尿液和粪便中排泄。为了确定适量饮酒对健康志愿者（n=41）整体尿液代谢组的影响，对一项红葡萄酒干预研究（每天 250 毫升，28 天）的样本进行了调查。在干预前后采集了 24 小时尿液，并通过非靶向超高效液相色谱-飞行时间质谱代谢组学方法进行了分析。鉴定出了 94 种与饮酒相关的化合物，包括特定的葡萄酒成分（酒石酸）、微生物衍生的酚类代谢物（5-（二羟基苯基）-γ-缬草酸和 4-羟基-5-（苯基）-戊酸）和内源性化合物。还讨论了粪便和尿液代谢组之间的一些平行关系。
