Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02473, Korea.
Institute of Life Science and Natural Resources, Korea University, Seoul 02473, Korea.
Mol Cells. 2018 Apr 30;41(4):351-361. doi: 10.14348/molcells.2018.2195. Epub 2018 Feb 27.
Sucrose is a crucial compound for the growth and development of plants, and the regulation of multiple genes depends on the amount of soluble sugars present. Sucrose acts as a signaling molecule that regulates a proton-sucrose symporter, with its sensor being the sucrose transporter. Flavonoid and anthocyanin biosynthesis are regulated by sucrose, and sucrose signaling can affect flavonoid and anthocyanin accumulation. In the present study, we found a Myb transcription factor affecting accumulation of anthocyanin. showed an increase in its expression in response to sucrose treatment. Under normal conditions, anthocyanin accumulation was similar between Col-0 (wild type) and mutant seedlings; however, under sucrose treatment, the level of anthocyanin accumulation was lower in the mutant plants than in Col-0 plants. Preliminary microarray analysis led to the investigation of the expression of one candidate gene, , in the mutant. The phosphate translocator, which is a plastidial phosphate antiporter family, catalyzes the import of glucose-6-phosphate (G-6-P) into the chloroplast. gene expression was altered in seedlings in a sucrosedependent manner in response to circadian cycle. Furthermore, the lack of resulted in altered accumulation of maltose in a sucrosedependent manner. Therefore, the sucrose responsive AtMyb56 regulates gene expression in a sucrose-dependent manner to modulate maltose and anthocyanin accumulations in response to the circadian cycle.
蔗糖是植物生长和发育的关键化合物，多种基因的调节依赖于存在的可溶性糖的量。蔗糖作为一种信号分子调节质子-蔗糖协同转运蛋白，其传感器是蔗糖转运蛋白。类黄酮和花青素的生物合成受蔗糖调节，蔗糖信号可以影响类黄酮和花青素的积累。在本研究中，我们发现了一个影响花青素积累的 Myb 转录因子。 对蔗糖处理的响应表现出表达增加。在正常条件下，Col-0（野生型）和 突变体幼苗之间的花青素积累相似；然而，在蔗糖处理下， 突变体植物中花青素的积累水平低于 Col-0 植物。初步的微阵列分析导致对候选基因 的表达进行研究， 在 突变体中。磷酸转运蛋白是质体磷酸盐反向转运蛋白家族，催化葡萄糖-6-磷酸（G-6-P）进入叶绿体。 基因的表达在 幼苗中以蔗糖依赖的方式被改变，以响应昼夜节律。此外， 的缺乏导致麦芽糖的积累以蔗糖依赖的方式发生改变。因此，蔗糖应答的 AtMyb56 以蔗糖依赖的方式调节 基因的表达，以调节麦芽糖和花青素在昼夜节律中的积累。
