Purena Rupal, Seth Rohit, Bhatt Renu
Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, Bilaspur, 495009, Chhattisgarh, India.
Department of Zoology, Guru Ghasidas Vishwavidyalaya, Bilaspur, 495009, Chhattisgarh, India.
Toxicol Rep. 2018 Feb 3;5:270-277. doi: 10.1016/j.toxrep.2018.01.008. eCollection 2018.
Nephrotoxicity is a major limiting factor in cisplatin treatment. In the present study hydro-ethanolic leaf extract of was investigated for its protective role in cisplatin induced nephrotoxicity. The experiment was designed for 14 days and male Wistar rats were divided into 9 groups (n = 5). Group 1 served as control (with no treatment), group 2 served as a vehicle control and received 0.9% NaCl intraperitoneally (i.p.) on 11th day of the treatment, group 3 received a single dose of cisplatin on 11th day (12 mg/kg body weight, i.p.), group 4-6 received leaf extract only (100 mg/kg, 200 mg/kg and 400 mg/kg body weight, respectively) throughout the treatment, group 7-9 received leaf extract (100 mg/kg, 200 mg/kg and 400 mg/kg body weight, respectively) throughout the treatment and single dose of cisplatin on the 11th day of the leaf extract treatment. At the end of the experiment ( on 14th day) blood samples were collected from all the groups and were sacrificed to study renal functional parameters. Treatment with above doses of leaf extract significantly (p ≤ 0.05) attenuates renal damage by decreasing serum creatinine and blood urea nitrogen (BUN), enhanced the activities of Catalase, SOD, GPx, GR and decreased the renal MDA level compared with the cisplatin treatment group. Furthermore the oral administration of Amla leaf extract improves histological damage and morphological changes in RBCs. Our results suggest that, leaf extract of may ameliorate renal damage caused by cisplatin.
肾毒性是顺铂治疗中的一个主要限制因素。在本研究中，对[植物名称]的水乙醇叶提取物在顺铂诱导的肾毒性中的保护作用进行了研究。实验设计为期14天，雄性Wistar大鼠分为9组（n = 5）。第1组作为对照组（未治疗），第2组作为溶剂对照组，在治疗的第11天腹腔注射（i.p.）0.9%氯化钠，第3组在第11天接受单剂量顺铂（12 mg/kg体重，i.p.），第4 - 6组在整个治疗过程中仅接受叶提取物（分别为100 mg/kg、200 mg/kg和400 mg/kg体重），第7 - 9组在整个治疗过程中接受叶提取物（分别为100 mg/kg、200 mg/kg和400 mg/kg体重），并在叶提取物治疗的第11天接受单剂量顺铂。在实验结束时（第14天），从所有组采集血样并处死大鼠以研究肾功能参数。与顺铂治疗组相比，用上述剂量的[植物名称]叶提取物治疗可显著（p≤0.05）减轻肾损伤，降低血清肌酐和血尿素氮（BUN），增强过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶的活性，并降低肾丙二醛水平。此外，印度醋栗叶提取物的口服给药改善了红细胞的组织学损伤和形态变化。我们的结果表明，[植物名称]叶提取物可能改善顺铂引起的肾损伤。
