Nasr Ashraf Y
Anatomy Department, Faculty of Medicine, Zagazig University, Egypt.
Toxicol Rep. 2014 Sep 16;1:682-691. doi: 10.1016/j.toxrep.2014.09.003. eCollection 2014.
Cisplatin (CP) is one of the most active cytotoxic drugs. However, it has several side effects that are associated with increased oxidative stress. Aged garlic extract (AGE) is a natural product containing different compounds with antioxidant activity. The present study aimed to evaluate the effect of AGE on CP-induced hepatotoxicity. Four equally male rat groups: control, AGE-treated (250 mg/kg once for 21 days), CP-treated (7.5 mg/kg, once intraperitoneal), combined AGE and CP-treated were used. Blood samples were collected to investigate blood picture and serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), bilirubin (TSB) and albumin. The liver of each rat was excised, cleaned, weighed, rinsed in ice-cold saline and homogenized for assessment malondialdehyde (MDA) level, catalase (CAT) and superoxide dismutase (SOD) activities and level of reduced glutathione (GSH). Histological examination was also carried out. AGE-pretreated rats revealed significant reduction in serum levels of AST, ALT & TSB and increase serum albumin level induced by CP administration. Furthermore, AGE significantly ameliorated CP-induced increase in MDA level and decrease in GSH level, CAT and SOD activities in liver tissue homogenates. Additionally, histopathological and blood picture examinations revealed markedly ameliorated CP-induced toxicity on blood cells parameters and liver structure. Our results prove that AGE has antioxidant and protective effects against CP-induced oxidative stress and changes in parameters of blood cells and liver structure in rats. Thus, it could be used as a dietary supplementation to reduce toxic side effects of anticancer drugs.
顺铂（CP）是最有效的细胞毒性药物之一。然而，它有几种与氧化应激增加相关的副作用。 aged garlic extract (AGE) 是一种含有具有抗氧化活性的不同化合物的天然产物。本研究旨在评估AGE对CP诱导的肝毒性的影响。使用了四个雄性大鼠组：对照组、AGE处理组（250mg/kg，单次给药，共21天）、CP处理组（7.5mg/kg，腹腔注射一次）、AGE和CP联合处理组。采集血样以研究血常规以及天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、胆红素（TSB）和白蛋白的血清水平。切除每只大鼠的肝脏，清洗、称重，在冰冷的盐水中冲洗并匀浆，以评估丙二醛（MDA）水平、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性以及还原型谷胱甘肽（GSH）水平。还进行了组织学检查。经AGE预处理的大鼠显示，CP给药诱导的AST、ALT和TSB血清水平显著降低，血清白蛋白水平升高。此外，AGE显著改善了CP诱导的肝脏组织匀浆中MDA水平升高以及GSH水平、CAT和SOD活性降低的情况。此外，组织病理学和血常规检查显示，CP诱导的对血细胞参数和肝脏结构的毒性明显改善。我们的结果证明，AGE对CP诱导的大鼠氧化应激以及血细胞参数和肝脏结构变化具有抗氧化和保护作用。因此，它可作为膳食补充剂用于减少抗癌药物的毒副作用。
