临床 MRI 扫描仪中的体内极化转移。
In vivo hyperpolarization transfer in a clinical MRI scanner.
机构信息
Department of Radiology and Biomedical Imaging, University of California, San Francisco, California.
GE Healthcare, Dallas, Texas.
出版信息
Magn Reson Med. 2018 Aug;80(2):480-487. doi: 10.1002/mrm.27154. Epub 2018 Feb 27.
PURPOSE
The purpose of this study was to investigate the feasibility of in vivo C-> H hyperpolarization transfer, which has significant potential advantages for detecting the distribution and metabolism of hyperpolarized C probes in a clinical MRI scanner.
METHODS
A standalone pulsed C RF transmit channel was developed for operation in conjunction with the standard H channel of a clinical 3T MRI scanner. Pulse sequences for C power calibration and polarization transfer were programmed on the external hardware and integrated with a customized water-suppressed H MRS acquisition running in parallel on the scanner. The newly developed RF system was tested in both phantom and in vivo polarization transfer experiments in J -coupled systems: phantom experiments in thermally polarized and hyperpolarized [2- C]glycerol, and H detection of [2- C]lactate generated from hyperpolarized [2- C]pyruvate in rat liver in vivo.
RESULTS
Operation of the custom pulsed C RF channel resulted in effective C-> H hyperpolarization transfer, as confirmed by the characteristic antiphase appearance of H-detected, J -coupled doublets. In conjunction with a pulse sequence providing 190-fold water suppression in vivo, H detection of hyperpolarized [2- C]lactate generated in vivo was achieved in a rat liver slice.
CONCLUSION
The results show clear feasibility for effective C-> H hyperpolarization transfer in a clinical MRI scanner with customized heteronuclear RF system.
目的
本研究旨在探讨体内 C->H 极化转移的可行性，这对于在临床 MRI 扫描仪中检测极化 C 探针的分布和代谢具有显著的潜在优势。
方法
开发了一个独立的脉冲 C RF 发射通道，与临床 3T MRI 扫描仪的标准 H 通道配合使用。C 功率校准和极化转移的脉冲序列在外部硬件上编程，并与在扫描仪上并行运行的定制水抑制 H MRS 采集集成。在热极化和高极化 [2-C]甘油的幻影实验以及在体大鼠肝内高极化 [2-C]丙酮酸产生的 [2-C]乳酸的 H 检测的 J 耦合系统中，对新开发的 RF 系统进行了测试。
结果
定制脉冲 C RF 通道的操作导致了有效的 C->H 极化转移，这通过 H 检测的特征反相信号证实，J 耦合双峰。与在体提供 190 倍水抑制的脉冲序列结合使用，在大鼠肝切片中实现了体内产生的高极化 [2-C]乳酸的 H 检测。
结论
结果表明，使用定制的异核 RF 系统在临床 MRI 扫描仪中进行有效的 C->H 极化转移是可行的。
Zotero 插件