Institute of Biomedical Research of Lleida IRBLleida, 25198, Lleida, Catalunya, Spain; Departament de Ciències Mèdiques Bàsiques, University of Lleida, 25198, Lleida, Catalunya, Spain.
Departament de Ciències Mèdiques Bàsiques, University of Lleida, 25198, Lleida, Catalunya, Spain.
Cell Rep. 2018 Feb 27;22(9):2421-2430. doi: 10.1016/j.celrep.2018.02.020.
Microorganisms evolved adaptive responses to survive stressful challenges in ever-changing environments. Understanding the relationships between the physiological/metabolic adjustments allowing cellular stress adaptation and gene expression changes being used by organisms to achieve such adjustments may significantly impact our ability to understand and/or guide evolution. Here, we studied those relationships during adaptation to various stress challenges in Saccharomyces cerevisiae, focusing on heat stress responses. We combined dozens of independent experiments measuring whole-genome gene expression changes during stress responses with a simplified kinetic model of central metabolism. We identified alternative quantitative ranges for a set of physiological variables in the model (production of ATP, trehalose, NADH, etc.) that are specific for adaptation to either heat stress or desiccation/rehydration. Our approach is scalable to other adaptive responses and could assist in developing biotechnological applications to manipulate cells for medical, biotechnological, or synthetic biology purposes.
微生物进化出适应性反应，以在不断变化的环境中应对充满压力的挑战。了解使细胞适应压力的生理/代谢调整与生物体用于实现这些调整的基因表达变化之间的关系，可能会显著影响我们理解和/或引导进化的能力。在这里，我们研究了在酿酒酵母适应各种压力挑战（重点是热应激反应）过程中的这些关系。我们将数十项独立的实验与一个简化的中心代谢动力学模型相结合，这些实验测量了应激反应过程中的全基因组基因表达变化。我们确定了模型中一组生理变量（例如 ATP、海藻糖、NADH 等的产生）的替代定量范围，这些范围是适应热应激或干燥/复水的特异性指标。我们的方法可扩展到其他适应性反应，并可协助开发生物技术应用，以用于医疗、生物技术或合成生物学目的来操纵细胞。
