Hu Yan-Ru, Wang Yue, Chen Yu-Jie, Chai Qian-Qian, Dong Hao-Zhe, Shen Jin-Wen, Qi Yuan-Cheng, Wang Feng-Qin, Wen Qing
Key Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.
Antioxidants (Basel). 2022 May 13;11(5):968. doi: 10.3390/antiox11050968.
(Jacq.) P. Kumm is cultivated worldwide, and its growth is seriously threatened by heat stress. Here, we performed a comprehensive analysis to investigate the influence of the phytohormone salicylic acid (SA) in under HS. The results showed that the hyphal growth recovery rate and the antioxidant capacity of increased with exogenous SA application (0.01 mmol/L and 0.05 mmol/L) after HS treatment. Metabolomic and transcriptomic analyses showed that SA application (0.05 mmol/L) weakened central carbon metabolism to allow cells to survive HS efficiently. In addition, SA shifted glycolysis to one-carbon metabolism to produce ROS scavengers (GSH and NADPH) and reduced ROS production by altering mitochondrial metabolism. SA also maintained nucleotide homeostasis, led to membrane lipid remodeling, activated the MAPK pathway, and promoted the synthesis of cell-wall components. This study provides a reference for further study of SA in microorganisms.
(Jacq.) P. Kumm在全球范围内种植，其生长受到热胁迫的严重威胁。在此，我们进行了一项全面分析，以研究植物激素水杨酸（SA）在热胁迫下的影响。结果表明，热胁迫处理后，外源施用SA（0.01 mmol/L和0.05 mmol/L）可提高其菌丝生长恢复率和抗氧化能力。代谢组学和转录组学分析表明，施用SA（0.05 mmol/L）会削弱中心碳代谢，使细胞能够有效应对热胁迫存活下来。此外，SA将糖酵解转变为一碳代谢，以产生活性氧清除剂（谷胱甘肽和还原型辅酶Ⅱ），并通过改变线粒体代谢减少活性氧的产生。SA还维持核苷酸稳态，导致膜脂重塑，激活丝裂原活化蛋白激酶途径，并促进细胞壁成分的合成。本研究为进一步研究SA在微生物中的作用提供了参考。
