Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Carretera a La Victoria Km. 0.6, Apartado 1735, Hermosillo, Sonora 83304, Mexico.
Nutrients. 2018 Feb 26;10(3):262. doi: 10.3390/nu10030262.
It has been reported that fermented milk (FM) with NRRL B-50571 had an antihypertensive effect in spontaneously hypertensive rats (SHR) and prehypertensive subjects. Therefore, the objective of the present study was to evaluate the possible mechanisms involved (angiotensin converting enzyme inhibition (ACEI), enhancement of nitric oxide production, antioxidant activity and opioid effect), in the antihypertensive effect of FM with SHR. First, twenty one SHR were randomized into three groups to either receive in a single-oral dose of purified water (negative control), FM, or naloxone (opioid receptor antagonist) + FM. In a parallel study, twenty seven SHR were randomized into three groups to either receive ad libitum purified water (negative control), Captopril or FM. After six weeks of treatment ACEI activity, enhancement of nitric oxide production, and antioxidant activity were evaluated in plasma. Results indicated that opioid receptors were not involved in the hypotensive effect of FM. However, ACEI activity (94 U/L), the oxidative stress index (malondialdehyde/catalase + glutathione peroxidase) 0.9, and nitric oxide in plasma (4.4 ± 1.3 U/L), were significantly different from the negative control, and not significantly different from the Captopril group. Thus, these results suggested that these mechanisms are involved in the hypotensive effect of FM.
据报道，NRRL B-50571 发酵乳对自发性高血压大鼠（SHR）和高血压前期患者具有降压作用。因此，本研究的目的是评估 FM 对 SHR 降压作用的可能机制（血管紧张素转换酶抑制（ACEI）、增强一氧化氮生成、抗氧化活性和阿片样作用）。首先，21 只 SHR 随机分为三组，分别给予单剂量纯化水（阴性对照）、FM 或纳洛酮（阿片受体拮抗剂）+FM。在平行研究中，27 只 SHR 随机分为三组，分别给予随意纯化水（阴性对照）、卡托普利或 FM。治疗 6 周后，评估血浆中 ACEI 活性、一氧化氮生成增强和抗氧化活性。结果表明，阿片受体不参与 FM 的降压作用。然而，ACEI 活性（94 U/L）、氧化应激指数（丙二醛/过氧化氢酶+谷胱甘肽过氧化物酶）0.9 和血浆中一氧化氮（4.4±1.3 U/L）与阴性对照组显著不同，与卡托普利组无显著差异。因此，这些结果表明这些机制参与了 FM 的降压作用。
