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在有无月桂枯萎病病原菌(劳雷尔散斑壳菌)的情况下,在人工培养基上饲养双刺材小蠹。

Xyleborus bispinatus Reared on Artificial Media in the Presence or Absence of the Laurel Wilt Pathogen (Raffaelea lauricola).

作者信息

Menocal Octavio, Cruz Luisa F, Kendra Paul E, Crane Jonathan H, Cooperband Miriam F, Ploetz Randy C, Carrillo Daniel

机构信息

Tropical Research & Education Center, University of Florida 18905 SW 280th St, Homestead, FL 33031, USA.

Subtropical Horticulture Research Station, USDA-ARS, 13601 Old Cutler Rd., Miami, FL 33158, USA.

出版信息

Insects. 2018 Feb 28;9(1):30. doi: 10.3390/insects9010030.

DOI:10.3390/insects9010030
PMID:29495585
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5872295/
Abstract

Like other members of the tribe Xyleborini, Eichhoff can cause economic damage in the Neotropics. has been found to acquire the laurel wilt pathogen (T. C. Harr., Fraedrich & Aghayeva) when breeding in a host affected by the pathogen. Its role as a potential vector of is under investigation. The main objective of this study was to evaluate three artificial media, containing sawdust of avocado ( Mill.) and silkbay ( Nash.), for rearing under laboratory conditions. In addition, the media were inoculated with to evaluate its effect on the biology of . There was a significant interaction between sawdust species and for all media. Two of the media supported the prolific reproduction of , but the avocado-based medium was generally more effective than the silkbay-based medium, regardless whether or not it was inoculated with . had a neutral or positive effect on beetle reproduction. The pathogen was frequently recovered from beetle galleries, but only from a few individuals which were reared on inoculated media, and showed limited colonization of the beetle's mycangia. Two media with lower water content were most effective for rearing .

摘要

与小蠹族的其他成员一样,艾氏小蠹会在新热带区造成经济损失。人们发现,当在受该病原菌影响的寄主中繁殖时,艾氏小蠹会感染月桂枯萎病原菌(T.C. 哈尔、弗雷德里克 & 阿加耶娃)。其作为该病原菌潜在传播媒介的作用正在研究中。本研究的主要目的是评估三种人工培养基,这些培养基含有鳄梨(米勒)和润楠(纳什)的锯末,用于在实验室条件下饲养艾氏小蠹。此外,在培养基中接种该病原菌以评估其对艾氏小蠹生物学特性的影响。对于所有培养基而言,锯末种类与该病原菌之间存在显著的相互作用。其中两种培养基支持艾氏小蠹大量繁殖,但无论是否接种该病原菌,以鳄梨为基础的培养基总体上比以润楠为基础的培养基更有效。该病原菌对甲虫繁殖具有中性或积极影响。病原菌经常从甲虫蛀道中分离出来,但仅从少数在接种培养基上饲养的个体中分离出来,并且在甲虫的菌囊中有有限的定殖。两种含水量较低的培养基对饲养艾氏小蠹最为有效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a1/5872295/c595b579e90a/insects-09-00030-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a1/5872295/19b03bbef2d0/insects-09-00030-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a1/5872295/c26aa640b185/insects-09-00030-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a1/5872295/c595b579e90a/insects-09-00030-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a1/5872295/19b03bbef2d0/insects-09-00030-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a1/5872295/c26aa640b185/insects-09-00030-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a1/5872295/c595b579e90a/insects-09-00030-g003.jpg

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