Romiszewski Przemysław, Kostro Krzysztof, Lisiecka Urszula
Animal Medical Center, 5255 York Rd, P.O. Box. 324, Holicong, PA, 18928, USA.
Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612, Lublin, Poland.
BMC Vet Res. 2018 Mar 5;14(1):70. doi: 10.1186/s12917-018-1383-6.
The aim of the present study was to assess the effects of subclinical inflammation on specific humoral immunity in dogs vaccinated with Nobivac® DHP based on serum levels of CRP and Hp. Dogs from the group I were administered Nobivac® DHP, the vaccine against distemper, infectious hepatitis and parvovirus whereas group II animals received subcutaneous turpentine oil to induce subclinical inflammation, followed by Nobivac® DHP after 24 h. Animals in group III received only turpentine oil in the way and amount identical to that as in group II.
Nobivac DHP relatively poorly induced the immune inflammatory response showing good immunogenic properties, which was evidenced by only a double increase in mean CRP and Hp levels associated with antigenic stimulation in group I. In group II, serum neutralization (SN) and haemagglutination inhibition (HI) results were quite closely correlated with serum levels of CPR and Hp.
Our findings suggest that the efficacy of vaccinations in dogs can be significantly affected by subclinical inflammations, which is indicated by a correlation between serum CRP and Hp levels versus antibody titres for canine distemper and parvovirus in both experimental groups of dogs (group I and II). The correlation of mean CRP and Hp values in dogs with subclinical inflammation and after vaccination with the kinetics of increasing antibody titres against distemper and parvovirus in group II dogs reflects the severity of inflammatory response and the extent of specific humoral immunity. Routine determinations of serum CRP and Hp levels as the indices of inflammation severity can be the essential biochemical markers for assessment of dogs' health in the period preceding specific immunoprophylaxis and efficacy of the vaccine.
本研究的目的是基于血清中CRP和Hp水平，评估亚临床炎症对接种Nobivac® DHP疫苗的犬类特异性体液免疫的影响。第一组犬接种Nobivac® DHP，这是一种预防犬瘟热、传染性肝炎和细小病毒的疫苗；而第二组动物皮下注射松节油以诱导亚临床炎症，24小时后接种Nobivac® DHP。第三组动物仅以与第二组相同的方式和剂量注射松节油。
Nobivac DHP诱导免疫炎症反应的能力相对较差，但显示出良好的免疫原性，这一点仅通过第一组中与抗原刺激相关的平均CRP和Hp水平仅增加两倍得到证明。在第二组中，血清中和（SN）和血凝抑制（HI）结果与CPR和Hp的血清水平密切相关。
我们的研究结果表明，亚临床炎症可显著影响犬类疫苗接种的效果，这在两个实验组犬（第一组和第二组）中血清CRP和Hp水平与犬瘟热和细小病毒抗体滴度之间的相关性中得到体现。第二组犬亚临床炎症及接种疫苗后平均CRP和Hp值与抗犬瘟热和细小病毒抗体滴度增加动力学之间的相关性反映了炎症反应的严重程度和特异性体液免疫的程度。作为炎症严重程度指标的血清CRP和Hp水平的常规测定，可能是评估犬在特异性免疫预防之前的健康状况以及疫苗效力的重要生化标志物。
