National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, 76080, Pakistan.
Institute of Biochemistry University of Sindh, Jamshoro, Pakistan.
Lipids Health Dis. 2018 Mar 5;17(1):36. doi: 10.1186/s12944-018-0683-y.
Hepatitis B virus (HBV) exerts an intense impact on host lipid metabolism. Hence the aim of present study is to determine metabolic derangement that occurred in subjects suffering from hepatitis B patients.
The fasting blood samples were collected from hepatitis B patients (n = 50) attended in Taluka hospital TandoAdam, Sindh with age and gender matched controls (n = 50). Serum lipid profile and fatty acid (FA) composition were analyzed by micro-lab and gas chromatography.
The hepatitis B patients have significantly lower level (p < 0.01) of lipid profile including total cholesterol (TC), triacylglyceride (TAG), high density lipoprotein-C (HDL-C) very low density lipoprotein-cholesterol (VLDL-C), low density lipoprotein-cholesterol (LDL-C), and total lipid (TL) in comparison to controls, indicating hypolipidemia in patients. The result of total FA composition of HBV patients in comparison to controls reveal that myristic, palmitic, docosahexaenoic acids were significantly (p < 0.05) higher, while linoleic, eicosatrienoic, arachidonic, eicosapentaenoic acids were lower in HBV patients in comparison to controls. The elongase, ∆5 and ∆6-desaturase enzymes activities were found lower, while ∆9-desaturase activity was higher in hepatitis B patients as compared to controls, which indicates the impaired lipid metabolism.
The serum saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) were increased while polyunsaturated fatty acid (PUFA) was reduced in both total and free form in hepatitis B patients due to altered activities of enzyme desaturases with impaired PUFA metabolism and non-enzymatic oxidation.
乙型肝炎病毒（HBV）对宿主脂质代谢有强烈影响。因此，本研究旨在确定乙型肝炎患者中发生的代谢紊乱。
从在信德省坦多阿当的塔鲁卡医院就诊的乙型肝炎患者（n=50）和年龄、性别匹配的对照组（n=50）中采集空腹血样。采用微实验室和气相色谱法分析血清脂质谱和脂肪酸（FA）组成。
乙型肝炎患者的脂质谱水平显著降低（p<0.01），包括总胆固醇（TC）、三酰甘油（TAG）、高密度脂蛋白-C（HDL-C）、极低密度脂蛋白胆固醇（VLDL-C）、低密度脂蛋白胆固醇（LDL-C）和总脂质（TL），表明患者存在脂质减少。与对照组相比，HBV 患者的总 FA 组成结果表明，豆蔻酸、棕榈酸、二十二碳六烯酸显著升高（p<0.05），而亚油酸、二十碳三烯酸、花生四烯酸、二十碳五烯酸在 HBV 患者中较低。与对照组相比，HBV 患者的延伸酶、Δ5 和 Δ6-去饱和酶活性较低，而 Δ9-去饱和酶活性较高，表明脂质代谢受损。
由于酶去饱和酶活性改变导致多不饱和脂肪酸（PUFA）代谢和非酶促氧化受损，乙型肝炎患者的总形式和游离形式的血清饱和脂肪酸（SFA）和单不饱和脂肪酸（MUFA）增加，而多不饱和脂肪酸（PUFA）减少。
