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基于萘的荧光探针用于谷胱甘肽及其在活细胞和脓毒症患者中的应用。

Naphthalene-based fluorescent probes for glutathione and their applications in living cells and patients with sepsis.

机构信息

Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea.

Severance Biomedical Science Institute, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea.

出版信息

Theranostics. 2018 Feb 3;8(5):1411-1420. doi: 10.7150/thno.22252. eCollection 2018.

DOI:10.7150/thno.22252
PMID:29507630
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5835946/
Abstract

Among the biothiols-related diseases, sepsis is defined as life-threatening organ dysfunction caused by a dysregulated host response to infection and can result in severe oxidative stress and damage to multiple organs. In this study, we aimed to develop a fluorescence chemosensor that can both detect GSH and further predict sepsis. In this study, two new naphthalene dialdehyde compounds containing different functional groups were synthesized, and the sensing abilities of these compounds towards biothiols and its applications for prediction of sepsis were investigated. Our study revealed that the newly developed probe 6-methoxynaphthalene-2, 3-dicarbaldehyde (MNDA) has two-photon is capable of detecting GSH in live cells with two-photon microscopy (TPM) under the excitation at a wavelength of 900 nm. Furthermore, two GSH detection probes naphthalene-2,3-dicarboxaldehyde (NDA) and 6-fluoronaphthalene-2,3-dicarbaldehyde (FNDA) not only can detect GSH in living cells, but also showed clinical significance for the diagnosis and prediction of mortality in patients with sepsis. These results open up a promising direction for further medical diagnostic techniques.

摘要

在与生物硫醇相关的疾病中，败血症被定义为宿主对感染的失调反应导致的危及生命的器官功能障碍，可导致严重的氧化应激和多个器官损伤。在这项研究中，我们旨在开发一种荧光化学传感器，既能检测 GSH，又能进一步预测败血症。在这项研究中，我们合成了两种含有不同官能团的萘二醛化合物，并研究了这些化合物对生物硫醇的传感能力及其在预测败血症中的应用。我们的研究表明，新开发的探针 6-甲氧基萘-2,3-二醛（MNDA）在 900nm 波长的激发下，通过双光子显微镜（TPM）具有双光子检测 GSH 的能力。此外，两种 GSH 检测探针萘-2,3-二醛（NDA）和 6-氟萘-2,3-二醛（FNDA）不仅可以在活细胞中检测 GSH，而且对败血症患者的诊断和死亡率预测具有临床意义。这些结果为进一步的医学诊断技术开辟了一个有前途的方向。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/d9f30a7a44b5/thnov08p1411g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/8682f57ab410/thnov08p1411g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/be0cb639bc2b/thnov08p1411g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/dc13a2dbdb24/thnov08p1411g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/9478df124718/thnov08p1411g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/6bbbf1227bb4/thnov08p1411g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/c24551a0fd49/thnov08p1411g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/b772797e6eeb/thnov08p1411g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/6ba6b64a3360/thnov08p1411g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/d9f30a7a44b5/thnov08p1411g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/8682f57ab410/thnov08p1411g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/be0cb639bc2b/thnov08p1411g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/dc13a2dbdb24/thnov08p1411g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/9478df124718/thnov08p1411g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/fe994ace52bb/thnov08p1411g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/6bbbf1227bb4/thnov08p1411g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/c24551a0fd49/thnov08p1411g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/b772797e6eeb/thnov08p1411g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/6ba6b64a3360/thnov08p1411g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/5835946/d9f30a7a44b5/thnov08p1411g010.jpg

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