Tsvetkov Philipp O, Tabouret Emeline, Roman Andrei Y, Romain Sylvie, Bequet Céline, Ishimbaeva Olga, Honoré Stéphane, Figarella-Branger Dominique, Chinot Olivier, Devred François
Aix-Marseille Université, CNRS, INP, Inst Neurophysiopathol, Faculté de Pharmacie de Marseille, 13385 Marseille, France.
AP-HM, Hôpital de la Timone, Service de Neuro-Oncologie, 13005 Marseille, France.
Oncotarget. 2018 Jan 25;9(10):9391-9399. doi: 10.18632/oncotarget.24317. eCollection 2018 Feb 6.
Glioblastoma is the most frequent and aggressive primary brain tumor in adults. Recently, a growing number of studies have shown that denaturation profile of plasma samples obtained by differential scanning calorimetry (DSC) can represent a signature of a disease. In this study, we analyzed for the first time the DSC denaturation profiles of the plasma from patients with recurrent glioblastoma (n=17). Comparison to the one of healthy individuals (n=10) and to already described profiles in others cancer showed clear differences suggesting that this DSC profile may constitute a signature of glioblastoma. Parameters extracted from these profiles were used for cluster analysis which revealed the existence of glioblastoma profile subgroups which correlated with prognostic factors. Moreover, we showed that the presence of circulating bevacizumab and carmustine did not alter this calorimetric signature of the disease, indicating that an evolution of the profile could be followed without being masked by ongoing systemic treatment. Thus, our results constitute a very promising proof of principle that a specific calorimetric profile could be detected in the plasma of glioblastoma patients. Moreover, we believe that our findings point to a potential easy-to-use non-invasive monitoring tool for glioblastoma patients.
胶质母细胞瘤是成人中最常见且侵袭性最强的原发性脑肿瘤。最近，越来越多的研究表明，通过差示扫描量热法（DSC）获得的血浆样本变性曲线可代表一种疾病特征。在本研究中，我们首次分析了复发性胶质母细胞瘤患者（n = 17）血浆的DSC变性曲线。与健康个体（n = 10）的血浆以及其他癌症中已描述的曲线进行比较，结果显示出明显差异，这表明该DSC曲线可能构成胶质母细胞瘤的一种特征。从这些曲线中提取的参数用于聚类分析，结果揭示了与预后因素相关的胶质母细胞瘤曲线亚组的存在。此外，我们表明循环中的贝伐单抗和卡莫司汀的存在并未改变该疾病的这种量热特征，这表明可以追踪曲线的变化，而不会被正在进行的全身治疗所掩盖。因此，我们的结果构成了一个非常有前景的原理证明，即可以在胶质母细胞瘤患者的血浆中检测到特定的量热曲线。此外，我们相信我们的发现指向了一种潜在的、易于使用的胶质母细胞瘤患者非侵入性监测工具。
