College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, China.
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China.
Poult Sci. 2018 May 1;97(5):1699-1705. doi: 10.3382/ps/pex414.
In poultry, fowl adenovirus (FAdV) and immunosuppressive virus co-infection is likely to cause decreased egg production, inclusion body hepatitis, and pericardial effusion syndrome. In this study, fowl adenovirus infection was found in parental and descendent generations of chickens. We used quantitative polymerase chain reaction (PCR) and dot blot hybridization to detect the infection of reticuloendotheliosis (REV), avian leukosis virus (ALV), and chicken infectious anemia virus (CIAV) in 480 plasma samples. The test samples were 34.58% FADV-positive, 22.29% REV-positive, 7.5% CAV-positive, and 0.63% ALV-positive. Sequence analysis showed that FADV belonged to serotype 7, which can cause inclusion body hepatitis. The ALV strain was ALV-A, in which the homology of gp85 gene and SDAU09C1 was 97.3%. The positive rate was lower because of the purification of avian leukemia, whereas the phylogenetic tree analysis of REV showed that the highest homology was with IBD-C1605, which was derived from a vaccine isolate. Through pathogen detection in poultry we present, to our knowledge, the first discovery of fowl adenovirus type 7 infection in parental chickens and found that there was co-infection of FAdV and several immunosuppressive viruses, such as the purified ALV and CIAV. This indicates that multiple infection of different viruses is ever-present, and more attention should be given in the diagnosis process.
在禽类中，禽腺病毒（FAdV）和免疫抑制性病毒的共同感染可能导致产蛋量下降、包涵体肝炎和心包积液综合征。在本研究中，发现了鸡的亲代和后代禽类腺病毒感染。我们使用定量聚合酶链反应（PCR）和斑点杂交来检测 480 份血浆样本中网状内皮增生症（REV）、禽白血病病毒（ALV）和鸡传染性贫血病毒（CIAV）的感染。检测样本中，FADV 阳性率为 34.58%，REV 阳性率为 22.29%，CAV 阳性率为 7.5%，ALV 阳性率为 0.63%。序列分析表明，FADV 属于能够引起包涵体肝炎的 7 型。ALV 株为 ALV-A，gp85 基因与 SDAU09C1 的同源性为 97.3%。由于禽白血病的净化，阳性率较低，而 REV 的系统进化树分析表明，与源自疫苗株的 IBD-C1605 的同源性最高。通过对家禽病原体的检测，我们首次发现了亲代鸡的 7 型禽腺病毒感染，并发现 FAdV 与几种免疫抑制性病毒如净化的 ALV 和 CIAV 存在共同感染。这表明不同病毒的多重感染是普遍存在的，在诊断过程中应给予更多关注。
