Alkobtawi Mansour, Ray Heather, Barriga Elias H, Moreno Mauricio, Kerney Ryan, Monsoro-Burq Anne-Helene, Saint-Jeannet Jean-Pierre, Mayor Roberto
UMR3347 Université Paris Sud-Paris Saclay, Institut Curie/CNRS/U1021 INSERM, Centre Universitaire bât, 110 91405 ORSAY Cedex, Paris, France.
Dept. of Cell, Developmental and Integrative Biology, The University of Alabama at Birmingham MCLM 338, 1918 University Dr. Birmingham, AL 35294, USA.
Dev Biol. 2018 Dec 1;444 Suppl 1(Suppl 1):S202-S208. doi: 10.1016/j.ydbio.2018.02.020. Epub 2018 Mar 6.
The neural crest is a multipotent population of cells that originates a variety of cell types. Many animal models are used to study neural crest induction, migration and differentiation, with amphibians and birds being the most widely used systems. A major technological advance to study neural crest development in mouse, chick and zebrafish has been the generation of transgenic animals in which neural crest specific enhancers/promoters drive the expression of either fluorescent proteins for use as lineage tracers, or modified genes for use in functional studies. Unfortunately, no such transgenic animals currently exist for the amphibians Xenopus laevis and tropicalis, key model systems for studying neural crest development. Here we describe the generation and characterization of two transgenic Xenopus laevis lines, Pax3-GFP and Sox10-GFP, in which GFP is expressed in the pre-migratory and migratory neural crest, respectively. We show that Pax3-GFP could be a powerful tool to study neural crest induction, whereas Sox10-GFP could be used in the study of neural crest migration in living embryos.
神经嵴是一群多能细胞，可分化为多种细胞类型。许多动物模型被用于研究神经嵴的诱导、迁移和分化，其中两栖动物和鸟类是使用最为广泛的系统。在小鼠、鸡和斑马鱼中研究神经嵴发育的一项重大技术进展是转基因动物的产生，在这些转基因动物中，神经嵴特异性增强子/启动子驱动荧光蛋白的表达以用作谱系追踪剂，或驱动修饰基因的表达以用于功能研究。不幸的是，目前尚未有用于研究神经嵴发育的关键模型系统——非洲爪蟾和热带爪蟾的此类转基因动物。在此，我们描述了两个转基因非洲爪蟾品系Pax3-GFP和Sox10-GFP的产生和特性，在这两个品系中，GFP分别在前迁移和迁移的神经嵴中表达。我们表明，Pax3-GFP可能是研究神经嵴诱导的有力工具，而Sox10-GFP可用于研究活胚胎中的神经嵴迁移。
