Department of Pharmacy, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, 201999, China.
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, 200433, China.
Anal Chim Acta. 2018 Jul 19;1014:64-70. doi: 10.1016/j.aca.2018.02.027. Epub 2018 Feb 20.
Antipsychotics are the drugs most often involved in drug poisoning cases, and therefore, therapeutic drug monitoring (TDM) is necessary for safe and effective medication administration of these drugs. In this study, a coffee ring effect-based surface-enhanced Raman spectroscopy (CRE-SERS) method was developed and successfully used to monitor antipsychotic poisoning by using urine samples for the first time. The established method exhibited excellent SERS performance since more hot spots were obtained in the "coffee ring". Using the optimized CRE-SERS method, the sensitivity was improved one order more than that of the conventional method with reasonable reproducibility. The antipsychotic drug clozapine (CLO) spiked into urine samples at 0.5-50 μg mL was quantitatively detected, at concentrations above the thresholds for toxicity. The CRE-SERS method allowed CLO and its metabolites to be ultimately distinguished from real poisoning urine samples. The coffee-ring effect would provide more opportunities for practical applications of the SERS-based method. The frequent occurrence of drug poisoning may have created a new area for the application of the CRE-SERS method. It is anticipated that the developed method will also have great potential for other drug poisoning monitoring.
抗精神病药是药物中毒病例中最常涉及的药物，因此，为了安全有效地使用这些药物，需要进行治疗药物监测 (TDM)。在这项研究中，开发了一种基于咖啡环效应的表面增强拉曼光谱 (CRE-SERS) 方法，并首次成功地用于监测尿液中的抗精神病药物中毒。所建立的方法表现出优异的 SERS 性能，因为在“咖啡环”中获得了更多的热点。使用优化的 CRE-SERS 方法，灵敏度比常规方法提高了一个数量级，且具有合理的重现性。在毒性阈值以上的浓度下，可定量检测到尿液样本中 0.5-50μg mL 的氯氮平 (CLO) 等抗精神病药物。CRE-SERS 方法允许 CLO 及其代谢物最终与真实中毒尿液样本区分开来。咖啡环效应将为基于 SERS 的方法的实际应用提供更多机会。药物中毒的频繁发生可能为 CRE-SERS 方法的应用开辟了一个新领域。预计该方法也将在其他药物中毒监测方面具有巨大潜力。
