Nicolaus Copernicus University in Torun, Faculty of Biology and Environmental Protection, Department of Environmental Microbiology and Biotechnology, Lwowska 1, 87-100 Torun, Poland.
Nicolaus Copernicus University in Torun, Faculty of Chemistry, Department of Chemistry of Biomaterials and Cosmetics, Gagarin 7, 87-100 Torun, Poland.
Mater Sci Eng C Mater Biol Appl. 2018 May 1;86:103-108. doi: 10.1016/j.msec.2018.01.005. Epub 2018 Jan 31.
In this study polymeric blends based on collagen, chitosan and hyaluronic acid in the form of thin films with the addition of gentamicin sulfate were obtained. Surface morphology of films was evaluated based on atomic force microscopy images. Surface free energy was measured and its polar and dispersive components were calculated. Moreover, oxygen and water vapor permeability through the material were measured as well as the water content in materials was studied. Thermal stability was determined by differential scanning calorimetry. Microbiological tests were performed to evaluate the diffusion of the drug from matrixes. The results showed that thin films based on collagen, chitosan and hyaluronic acid enriched in gentamicin sulfate inhibit the growth of both, Gram negative bacteria (E. coli and P. aeruginosa) and Gram positive ones (S. aureus).
在这项研究中，制备了以明胶、壳聚糖和透明质酸为基质的聚合物共混物薄膜，并添加硫酸庆大霉素。通过原子力显微镜图像评估了薄膜的表面形貌。测量了表面自由能，并计算了其极性和分散分量。此外，还测量了材料的氧气和水蒸气透过率以及材料的含水量。通过差示扫描量热法确定了热稳定性。进行了微生物测试以评估药物从基质中的扩散情况。结果表明，富含硫酸庆大霉素的明胶、壳聚糖和透明质酸基薄膜能够抑制革兰氏阴性菌（大肠杆菌和铜绿假单胞菌）和革兰氏阳性菌（金黄色葡萄球菌）的生长。
