Department of Periodontology, Faculty of Dentistry, Khon Kaen University, Khon Kaen, Thailand.
Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Photodiagnosis Photodyn Ther. 2018 Jun;22:101-105. doi: 10.1016/j.pdpdt.2018.03.001. Epub 2018 Mar 10.
Curcumin, one of an established curcuminoid substances extracted from Curcuma longa, has been used as a photosensitizer (PS) in photodynamic therapy (PDT). Curcuminoid substances has been reported to have benefits in treating dental chronic infection and inflammation diseases, such as chronic periodontitis. The purpose of this study was to find the optimum concentration of Curcuma longa (CL) extract, containing all curcuminoid substances, and the power density of blue light (BL) in photodynamic therapy against periodontally pathogenic bacteria, A. actinomycetemcomitans.
Antibacterial activity of various concentrations of CL extract against A. actinomycetemcomitans was determined. Exponentially growing bacteria were combined with 2-fold dilution of CL extract solution ranging from 25 to 0.098 μg/ml. Co-culture bacteria treated with 0.12% chlorhexidine (CHX) served as the positive control. The effect of photostimulation with light emitting diode (LED) 420-480 nm at 16.8 J/cm for 1 min on the selected concentration of CL extract was examined. Bacteria viability was determined by plate counting technique. In addition, production of free radicals was tested by electron spin resonance spectroscope (ESR) with 5,5-dimethyl-1-pyrroline N-oxide (DMPO).
The antibacterial activity of CL extract was dose dependent. Without BL, 25 μg/ml CL extract showed 6.03 ± 0.39 logA. actinomycetemcomitans. Interestingly, the combination of BL and 0.78 μg/ml CL extract solution showed complete absence of A. actinomycetemcomitans. Peak signal intensity of hydroxyl radical production was also detected with the combination of BL and CL.
CL extract not only had antimicrobial activity but also could be used as an effective PS when stimulated with BL in PDT. The optimal antibacterial effect of CL extract with BL was equal to the standard oral disinfectant, 0.12% CHX.
姜黄素是从姜黄中提取的一种已被确定的姜黄素物质，已被用作光动力疗法（PDT）的光敏剂（PS）。姜黄素物质已被报道在治疗口腔慢性感染和炎症性疾病方面具有益处，如慢性牙周炎。本研究的目的是找到最佳浓度的姜黄（CL）提取物，其中包含所有姜黄素物质，以及针对牙周病病原体伴放线放线杆菌的蓝光（BL）的功率密度。
测定不同浓度的 CL 提取物对伴放线放线杆菌的抗菌活性。将指数生长期的细菌与 2 倍稀释的 CL 提取物溶液（浓度范围为 25 至 0.098μg/ml）混合。以 0.12%洗必泰（CHX）作为阳性对照。用 420-480nm 发光二极管（LED）光照射 1 分钟，光能量为 16.8J/cm，研究选定浓度的 CL 提取物的光刺激效果。用平板计数技术测定细菌活力。此外，用电子自旋共振光谱仪（ESR）用 5,5-二甲基-1-吡咯啉 N-氧化物（DMPO）测试自由基的产生。
CL 提取物的抗菌活性呈剂量依赖性。没有 BL，25μg/ml CL 提取物对伴放线放线杆菌的杀菌率为 6.03±0.39log。有趣的是，BL 和 0.78μg/ml CL 提取物溶液的组合使伴放线放线杆菌完全消失。用 BL 和 CL 组合也检测到羟基自由基产生的峰值信号强度。
CL 提取物不仅具有抗菌活性，而且在 PDT 中用 BL 刺激时也可以用作有效的 PS。CL 提取物与 BL 的最佳抗菌效果与标准口腔消毒剂 0.12%CHX 相当。
