一种使用含咪唑阳离子自由基引发剂制备的细胞靶向非细胞毒性荧光纳米凝胶温度计。
A Cell-Targeted Non-Cytotoxic Fluorescent Nanogel Thermometer Created with an Imidazolium-Containing Cationic Radical Initiator.
作者信息
Uchiyama Seiichi, Tsuji Toshikazu, Kawamoto Kyoko, Okano Kentaro, Fukatsu Eiko, Noro Takahiro, Ikado Kumiko, Yamada Sayuri, Shibata Yuka, Hayashi Teruyuki, Inada Noriko, Kato Masaru, Koizumi Hideki, Tokuyama Hidetoshi
机构信息
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033, Japan.
Central Laboratories for Key Technologies, KIRIN Company Limited, 1-13-5 Fukuura Kanazawa-ku, Yokohama, Kanagawa, 236-0004, Japan.
出版信息
Angew Chem Int Ed Engl. 2018 May 4;57(19):5413-5417. doi: 10.1002/anie.201801495. Epub 2018 Apr 6.
A cationic fluorescent nanogel thermometer based on thermo-responsive N-isopropylacrylamide and environment-sensitive benzothiadiazole was developed with a new azo compound bearing imidazolium rings as the first cationic radical initiator. This cationic fluorescent nanogel thermometer showed an excellent ability to enter live mammalian cells in a short incubation period (10 min), a high sensitivity to temperature variations in live cells (temperature resolution of 0.02-0.84 °C in the range 20-40 °C), and remarkable non-cytotoxicity, which permitted ordinary cell proliferation and even differentiation of primary cultured cells.
基于热响应性N-异丙基丙烯酰胺和环境敏感性苯并噻二唑，开发了一种阳离子荧光纳米凝胶温度计，其中一种带有咪唑环的新型偶氮化合物用作首个阳离子自由基引发剂。这种阳离子荧光纳米凝胶温度计在短时间孵育（10分钟）内显示出进入活的哺乳动物细胞的优异能力，对活细胞中的温度变化具有高灵敏度（在20-40°C范围内温度分辨率为0.02-0.84°C），并且具有显著的无细胞毒性，这使得普通细胞能够增殖，甚至原代培养细胞能够分化。
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