Pérez-Martínez Z, Álvarez-Argüelles M E, Rojo-Alba S, Castello-Abietar C, Boga J A, Morilla-Morilla A, Vivanco-Allende A, Rodríguez-Suárez J, Alonso-Álvarez M A, Melón S
Service of Microbiology, Hospital Universitario Central de Asturias (HUCA) and Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Service of Pediatric, Hospital Universitario Central de Asturias (HUCA), Oviedo, Spain.
Eur J Clin Microbiol Infect Dis. 2021 Oct;40(10):2185-2190. doi: 10.1007/s10096-021-04275-6. Epub 2021 May 13.
Enteroviruses (EV) have been linked to lymphocytic meningitis and exanthems, but they may also be involved in acute gastroenteritis (AGE), a condition whose aetiological agent often remains unidentified. In this work 1214 samples from individuals with AGE were studied with the aim of establishing the incidence of EV. The samples were collected between September and December in three different years and subjected to real-time genomic amplification in order to determine the viral load (VL). Of the 1214 samples studied, infection by a single virus was found in 328 cases (27%) and coinfection in 69 (5.7%). While adenoviruses (AdV) were the most frequent (14.8% of total), EV were present in 126 (10.4%) of the individuals tested. Of the 126 EV-positive samples, this virus was found as a single infection and coinfection in 76 (6.3%) and 50 (4.1%) cases, respectively. VL for EV was 5.58±1.51 log copies/ml (range 3.73-9.69) in the former and 6.27±1.75 (range 3.73-10.5) (p=0.02) in the latter. EV were identified in 97 children under 5 (16.9%) and in 29 (4.5%) patients over 5. Patients less than 5 years showed a higher VL that those more than 5 years age [6.08±1.57 (range 3.82-9.69) vs. 5.07±1.53 (range 3.73-10.58); (p=0.002)]. There was a high incidence of EV in AGE patients, and they were more frequent in those under 5, where they were found to replicate more efficiently. These results therefore indicate that testing for EV should be included in the diagnosis of AGE.
肠道病毒（EV）与淋巴细胞性脑膜炎和皮疹有关，但它们也可能参与急性胃肠炎（AGE），而这种疾病的病原体往往仍未明确。在这项研究中，对1214例急性胃肠炎患者的样本进行了研究，目的是确定肠道病毒的发病率。这些样本在三年中的9月至12月期间采集，并进行实时基因组扩增以确定病毒载量（VL）。在研究的1214个样本中，328例（27%）发现单一病毒感染，69例（5.7%）发现合并感染。虽然腺病毒（AdV）最为常见（占总数的14.8%），但在126例（10.4%）检测个体中发现了肠道病毒。在126份肠道病毒阳性样本中，该病毒分别在76例（6.3%）和50例（4.1%）中作为单一感染和合并感染被发现。前者肠道病毒的病毒载量为5.58±1.51 log拷贝/毫升（范围3.73 - 9.69），后者为6.27±1.75（范围3.73 - 10.5）（p = 0.02）。在97名5岁以下儿童（16.9%）和29名5岁以上患者（4.5%）中发现了肠道病毒。5岁以下患者的病毒载量高于5岁以上患者[6.08±1.57（范围3.82 - 9.69）对5.07±1.53（范围3.73 - 10.58）；（p = 0.002）]。急性胃肠炎患者中肠道病毒的发病率较高，在5岁以下儿童中更为常见，且发现它们复制更有效。因此，这些结果表明，在急性胃肠炎的诊断中应包括肠道病毒检测。
