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利用含有酰高丝氨酸内酯(AHLs)的上清液实现快速氨氧化菌(anammox)启动。

Fast start-up anammox process using Acyl-homoserine lactones (AHLs) containing supernatant.

机构信息

Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China; School of Food and Environment, Dalian University of Technology, Panjin 124000, China.

Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.

出版信息

J Environ Sci (China). 2018 Mar;65:127-132. doi: 10.1016/j.jes.2017.03.025. Epub 2017 Mar 29.

Abstract

N-dodecanoyl homoserine lactone (C-HSL) was detected in the supernatant of an anammox granular sludge reactor (AGSR). C-HSL could enhance the specific anammox activity of anammox biomass. Adding C-HSL-containing AGSR supernatant into the continuously stirred tank reactors reduced the start-up time of the anammox process from 80 to 66days. Moreover, the nitrogen loading rate was also enhanced to 1.6 times that of the control reactor. AHLs could increase the secretion of extracellular polymeric substances and anammox obtained better enrichment with the addition of AHLs-containing AGSR supernatant. Denaturing gradient gel electrophoresis analysis further revealed that AHLs played a role in mediating microbial community parameters. In conclusion, adding AHL-containing supernatant could be an effective and economical way to accelerate the start-up of anammox.

摘要

十二烷酰基高丝氨酸内酯(C-HSL)在厌氧氨氧化颗粒污泥反应器(AGSR)的上清液中被检测到。C-HSL 可以增强厌氧氨氧化生物量的特定厌氧氨氧化活性。将含有 C-HSL 的 AGSR 上清液添加到连续搅拌槽式反应器中,将厌氧氨氧化过程的启动时间从 80 天缩短至 66 天。此外,氮负荷率也提高到对照反应器的 1.6 倍。AHL 可以增加胞外聚合物的分泌,并且随着添加含有 AHL 的 AGSR 上清液,厌氧氨氧化获得了更好的富集。变性梯度凝胶电泳分析进一步表明,AHL 在调节微生物群落参数方面发挥作用。总之,添加含有 AHL 的上清液可能是一种有效且经济的方法,可以加速厌氧氨氧化的启动。

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