Department of Materials Science and Engineering, University of Connecticut, Storrs, CT 06269-3136, USA.
Department of Physics, University of Connecticut, Storrs, CT 06269-3046, USA.
Mater Sci Eng C Mater Biol Appl. 2018 Jun 1;87:112-119. doi: 10.1016/j.msec.2018.02.018. Epub 2018 Feb 24.
A detailed magnetization study, along with an assessment of the cellular proliferation, has been carried out on transition-metal-doped hydroxyapatite (HA), CaM(PO)(OH), where M = Mn, Co, and Fe. In particular, a series of MnHA powder samples with an x value of 0.04 ≤ x ≤ 1.21, one CoHA (x = 0.48) and one FeHA sample (x = 1.06) were synthesized using a wet chemical method along with an ion-exchange procedure. Characterization by transmission electron microscope (TEM), energy-dispersive X-ray spectroscopy (EDXS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) indicated that the substitution of M elements does not change the morphology and crystalline structure of pure HA that showing a single phased HA nano-rod. In every case, the magnetization isotherms for 10 K ≤ T ≤ 300 K were linear through the origin characteristic of a paramagnetic response with no indication of superparamagnetic behavior, hysteresis, or magnetic ordering. The magnetic behavior for all samples could be fit to the Curie-Weiss law yielding values for the M ion magnetic moments. The Mn magnetic moments were close to the spin-only value of S = 5/2 or 5.92 μ, while the Co moment (4.41 μ) was larger than the spin-only value for S = 3/2, indicating an orbital contribution due to incomplete quenching. The magnetic behavior for the FeHA sample showed a possible spin-state transition. In addition, no statistically significant differences were observed when cells were treated with the same dose of HA or MnHA up to 50 μg/mL, suggesting that the substituted Mn introduces no cytotoxicity to the HA powders.
对过渡金属掺杂羟基磷灰石（HA），CaM（PO）（OH），其中 M = Mn，Co 和 Fe 进行了详细的磁化研究，并评估了细胞增殖。特别是，通过湿化学方法和离子交换程序合成了一系列 MnHA 粉末样品，其中 x 值为 0.04≤x≤1.21，一个 CoHA（x=0.48）和一个 FeHA 样品（x=1.06）。透射电子显微镜（TEM），能量色散 X 射线光谱（EDXS），X 射线衍射（XRD）和傅里叶变换红外光谱（FTIR）的特征表明，M 元素的取代不会改变纯 HA 的形态和晶体结构，呈现出单相 HA 纳米棒。在每种情况下，10 K≤T≤300 K 的磁化等温线均通过原点呈线性，表现出顺磁响应的特征，没有超顺磁行为，磁滞或磁序的迹象。所有样品的磁行为都可以拟合居里-外斯定律，得出 M 离子磁矩的值。Mn 磁矩接近自旋-only 值 S=5/2 或 5.92 μ，而 Co 磁矩（4.41 μ）大于自旋-only 值 S=3/2，表明由于不完全淬灭导致轨道贡献。FeHA 样品的磁行为显示出可能的自旋态转变。此外，当细胞用相同剂量的 HA 或 MnHA 处理至 50μg/mL 时，没有观察到统计学上的显着差异，这表明取代的 Mn 不会对 HA 粉末产生细胞毒性。
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