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硒和锶共取代羟基磷灰石的合成、表征、抗菌性能及体外研究。

Synthesis, Characterization, Antibacterial Properties, and In Vitro Studies of Selenium and Strontium Co-Substituted Hydroxyapatite.

机构信息

Institute of Biomaterials, University of Erlangen-Nuremberg, 91058 Erlangen, Germany.

Lucideon Ltd., Penkhull, Stoke-on-Trent, Staffordshire ST4 7LQ, UK.

出版信息

Int J Mol Sci. 2021 Apr 19;22(8):4246. doi: 10.3390/ijms22084246.

DOI:10.3390/ijms22084246
PMID:33921909
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8072711/
Abstract

In this study, as a measure to enhance the antimicrobial activity of biomaterials, the selenium ions have been substituted into hydroxyapatite (HA) at different concentration levels. To balance the potential cytotoxic effects of selenite ions (SeO) in HA, strontium (Sr) was co-substituted at the same concentration. Selenium and strontium-substituted hydroxyapatites (Se-Sr-HA) at equal molar ratios of Se/(Se + P) and Sr/(Sr + Ca) at ( = 0, 0.01, 0.03, 0.05, 0.1, and 0.2) were synthesized via the wet precipitation route and sintered at 900 °C. The effect of the two-ion concentration on morphology, surface charge, composition, antibacterial ability, and cell viability were studied. X-ray diffraction verified the phase purity and confirmed the substitution of selenium and strontium ions. Acellular in vitro bioactivity tests revealed that Se-Sr-HA was highly bioactive compared to pure HA. Se-Sr-HA samples showed excellent antibacterial activity against both Gram-negative () and Gram-positive () bacterial strains. In vitro cell-material interaction, using human osteosarcoma cells MG-63 studied by WST-8 assay, showed that Se-HA has a cytotoxic effect; however, the co-substitution of strontium in Se-HA offsets the negative impact of selenium and enhanced the biological properties of HA. Hence, the prepared samples are a suitable choice for antibacterial coatings and bone filler applications.

摘要

在这项研究中，作为增强生物材料抗菌活性的一种措施，已将硒离子以不同浓度水平取代到羟基磷灰石（HA）中。为了平衡亚硒酸盐（SeO）在 HA 中的潜在细胞毒性作用，以相同浓度共取代了锶（Sr）。通过湿沉淀法合成了等摩尔比的硒（Se）/（Se + P）和锶（Sr）/（Sr + Ca）（ = 0、0.01、0.03、0.05、0.1 和 0.2）的硒-锶取代羟基磷灰石（Se-Sr-HA），并在 900°C 下烧结。研究了两种离子浓度对形貌、表面电荷、组成、抗菌能力和细胞活力的影响。X 射线衍射证实了相纯度，并证实了硒和锶离子的取代。非细胞体外生物活性测试表明，与纯 HA 相比，Se-Sr-HA 具有更高的生物活性。Se-Sr-HA 样品对革兰氏阴性（）和革兰氏阳性（）细菌菌株均表现出优异的抗菌活性。通过 WST-8 测定法用人骨肉瘤细胞 MG-63 研究体外细胞-材料相互作用表明，Se-HA 具有细胞毒性作用；然而，Sr 在 Se-HA 中的共取代抵消了硒的负面影响，并增强了 HA 的生物学特性。因此，所制备的样品是抗菌涂层和骨填充剂应用的合适选择。

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