Silkworm Physiology Laboratory, Central Tasar Research and Training Institute (Central Silk Board), P.O. Piska-Nagri, Ranchi 835303, Jharkhand, India.
Silkworm Physiology Laboratory, Central Tasar Research and Training Institute (Central Silk Board), P.O. Piska-Nagri, Ranchi 835303, Jharkhand, India.
Int J Biol Macromol. 2018 Jul 15;114:1102-1108. doi: 10.1016/j.ijbiomac.2018.03.058. Epub 2018 Mar 14.
The present study investigates the properties of sericin extracted from tasar silk fiber waste (TSFW). The surface morphology of TSFW was observed by scanning electron microscope (SEM). SEM images revealed the removal of residual sericin over the surface of TSFW. The molecular weight distribution of sericin was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results suggested that TSFW sericin represented a family of proteins with wide-ranging molecular weight distribution (11-245 kDa). Structural determination by FTIR revealed the presence of both α-helical and β-sheet structures. The colour was studied by colorimeter indicating less brightness, more red and yellow colour intensities. The carbon: nitrogen ratio (C:N) was studied by CHNS element analyzer and the ratio is 5.15-7.85. Thermal properties of TSFW sericin have been studied by thermogravimetric analysis (TGA) method. TGA curve showed higher thermal stability and variable degradation profiles. Furthermore, TSFW sericin contains 17 amino acids where serine, aspartic acid and glycine are the more significant compounds (54.34-60.49%). In addition, sericin was found to inhibit tyrosinase, elastase and glutathione-S-transferase activity, and had apparent radical scavenging impacts on 2.2‑diphenyl‑1‑picryl‑hydrazil (DPPH), hydrogen peroxide and inhibition of lipid peroxidation. Result suggested that TSFW sericins might be a valuable ingredient for cosmoceutical products.
本研究探讨了从塔斯马尼亚丝纤维废料（TSFW）中提取的丝胶的性质。通过扫描电子显微镜（SEM）观察 TSFW 的表面形态。SEM 图像显示 TSFW 表面的残余丝胶已被去除。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）检查丝胶的分子量分布。结果表明，TSFW 丝胶代表了一组具有广泛分子量分布（11-245 kDa）的蛋白质家族。傅里叶变换红外光谱（FTIR）结构测定表明存在α-螺旋和β-折叠结构。用分光光度计研究颜色，表明亮度较低，红色和黄色强度较大。通过 CHNS 元素分析仪研究碳：氮比（C：N），比值为 5.15-7.85。通过热重分析（TGA）法研究 TSFW 丝胶的热性能。TGA 曲线显示出较高的热稳定性和可变的降解曲线。此外，TSFW 丝胶含有 17 种氨基酸，其中丝氨酸、天冬氨酸和甘氨酸是更重要的化合物（54.34-60.49%）。此外，丝胶被发现抑制酪氨酸酶、弹性蛋白酶和谷胱甘肽-S-转移酶的活性，并且对 2.2-二苯基-1-苦基肼（DPPH）、过氧化氢和抑制脂质过氧化具有明显的自由基清除作用。结果表明，TSFW 丝胶可能是化妆品产品的有价值成分。
