Koc-Bilican Behlul, Karaduman-Yesildal Tugce, Tornaci Selay, Cansaran-Duman Demet, Oner Ebru Toksoy, Gül Serkan, Kaya Murat
Department of Molecular Biology and Genetics, Faculty of Science and Letters, Aksaray University, 68100 Aksaray, Türkiye.
Department of Bioengineering, Faculty of Engineering, Marmara University, 34469 Istanbul, Türkiye.
Polymers (Basel). 2025 Jul 27;17(15):2046. doi: 10.3390/polym17152046.
Extensive research on amphibians has focused on areas such as morphological and molecular taxonomy, ecology, embryology, and molecular phylogeny. However, the structure and biotechnological potential of egg jelly-which plays a protective and nutritive role for embryos-have remained largely unexplored. This study presents, for the first time, a detailed physicochemical analysis of the egg jelly of , an amphibian species, using Fourier Transform Infrared Spectroscopy, Thermogravimetric Analyzer, X-ray Diffraction, and elemental analysis. The carbohydrate content was determined via High-Performance Liquid Chromatography analysis, and the protein content was identified using Liquid Chromatography-Tandem Mass Spectrometry analysis. Additionally, it was revealed that this jelly exhibits a significant cytotoxic effect on melanoma cells (viability < 30%) while showing no cytotoxicity on healthy dermal fibroblast cells (viability > 70%). Consequently, this non-toxic, biologically derived, and cultivable material is proposed as a promising candidate for cancer applications, paving the way for further research in the field.
对两栖动物的广泛研究集中在形态学和分子分类学、生态学、胚胎学以及分子系统发育等领域。然而，对于胚胎具有保护和营养作用的卵胶的结构和生物技术潜力，在很大程度上仍未得到探索。本研究首次使用傅里叶变换红外光谱、热重分析仪、X射线衍射和元素分析，对一种两栖动物物种的卵胶进行了详细的物理化学分析。通过高效液相色谱分析测定碳水化合物含量，并使用液相色谱-串联质谱分析鉴定蛋白质含量。此外，研究发现这种卵胶对黑色素瘤细胞具有显著的细胞毒性作用（存活率<30%），而对健康的皮肤成纤维细胞没有细胞毒性（存活率>70%）。因此，这种无毒、生物来源且可培养的材料被认为是癌症应用的一个有前景的候选物，为该领域的进一步研究铺平了道路。
