N'Guessan Kombo F, Boyce Ceejay, Kwara Awewura, Archampong Timothy N A, Lartey Margaret, Sagoe Kwamena W, Kenu Ernest, Obo-Akwa Adjoa, Blackard Jason T
Division of Digestive Diseases, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
College of Medicine and Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA.
Virus Genes. 2018 Jun;54(3):361-367. doi: 10.1007/s11262-018-1555-2. Epub 2018 Mar 17.
Human pegivirus (HPgV) is a positive single-stranded RNA virus in the Flaviviridae family. Phylogenetic analysis reveals the presence of multiple HPgV genotypes with distinct geographic locations. HPgV is of interest because of its potential beneficial impact on HIV disease progression. Despite this, the effects of HPgV in the context of other viral infections, such as hepatitis B virus (HBV), are poorly understood, and data from resource-limited settings are scarce. Therefore, we conducted a cross-sectional analysis of HPgV in HIV/HBV co-infected patients in Ghana. Sera from 100 HIV/HBV co-infected individuals were evaluated for HPgV RNA, and the genotype determined by sequencing the 5' untranslated region. HPgV RNA was detected in 27 samples (27%). Of these, 26 were genotyped successfully with 23 belonging to HPgV genotype 1 and 3 belonging to HPgV genotype 2. The presence of HPgV RNA had no statistically significant impact on CD4 cell count or HBV DNA titers in the HIV/HBV co-infected patients. However, there was a trend towards decreased HBV DNA levels in HPgV RNA-positive patients with CD4 cell count < 200 (p = 0.0626). HPgV co-infection is common in Ghana. The effect of HPgV on HIV or HBV disease among HIV/HBV co-infected patients was minimal. However, decreased HBV DNA levels in HPgV RNA-positive patients with low CD4 cell counts highlight the need for prospective studies of HPgV in HIV and hepatitis co-infected patients, especially in those with advanced HIV disease, to study further the effects of HPgV on liver disease.
人pegivirus（HPgV）是黄病毒科的一种正单链RNA病毒。系统发育分析显示存在多个具有不同地理位置的HPgV基因型。由于其对HIV疾病进展具有潜在的有益影响，HPgV备受关注。尽管如此，人们对HPgV在其他病毒感染（如乙型肝炎病毒（HBV））背景下的影响了解甚少，且来自资源有限地区的数据也很匮乏。因此，我们对加纳HIV/HBV合并感染患者中的HPgV进行了横断面分析。对100例HIV/HBV合并感染个体的血清进行HPgV RNA检测，并通过对5'非翻译区进行测序来确定基因型。在27个样本（27%）中检测到HPgV RNA。其中，26个成功进行了基因分型，23个属于HPgV基因型1，3个属于HPgV基因型2。在HIV/HBV合并感染患者中，HPgV RNA的存在对CD4细胞计数或HBV DNA滴度没有统计学上的显著影响。然而，在CD4细胞计数<200的HPgV RNA阳性患者中，HBV DNA水平有下降趋势（p = 0.0626）。HPgV合并感染在加纳很常见。HPgV对HIV/HBV合并感染患者的HIV或HBV疾病的影响很小。然而，CD4细胞计数低的HPgV RNA阳性患者中HBV DNA水平降低，凸显了对HIV和肝炎合并感染患者，尤其是晚期HIV疾病患者进行HPgV前瞻性研究的必要性，以进一步研究HPgV对肝脏疾病的影响。
