Di Dalin, Chen Lei, Guo Yingying, Wang Lina, Wang Huidong, Ju Jiyu
Department of Immunology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.
Department of Hematology, The Hospital Affiliated to Weifang Medical University, Weifang, Shandong 261053, P.R. China.
Oncol Lett. 2018 Apr;15(4):5020-5026. doi: 10.3892/ol.2018.7972. Epub 2018 Feb 6.
Breast cancer suppressor candidate-1 (BCSC-1) is a candidate tumor suppressor gene that was identified recently. Decreased levels of BCSC-1 have been detected in a variety of cancer types in previous studies. Matrix metalloproteinase (MMP)-14 is a membrane-type MMP that plays an important role in tumor progression and prognosis. Previous research has indicated that MMP-14 is highly expressed in different cancer types and promotes tumor invasion or metastasis by remodeling the extracellular matrix. However, there have been few reports on BCSC-1 and MMP-14 in human breast cancer in recent years. In the present study, the association of BCSC-1 and MMP-14 with human breast cancer was investigated. The immunohistochemical analysis results revealed reduced expression of BCSC-1 and overexpression of MMP-14 in breast cancer tissues compared with adjacent normal breast tissues. Quantitative polymerase chain reaction and western blot analyses also showed that BCSC-1 was expressed at significantly lower levels, and that MMP-14 was expressed at significantly higher levels in breast cancer tissues compared with healthy breast tissue. Furthermore, decreased expression of BCSC-1 and overexpression of MMP-14 were associated with tumor cellular differentiation, lymph node metastasis and distant metastasis. A correlational analysis between BCSC-1 and MMP-14 was also conducted, and the results indicated a negative correlation between the two. In conclusion, the current findings indicate that BCSC-1 is downregulated, while MMP-14 is overexpressed in human breast cancer. These two genes may play important roles during the process of human breast cancer development.
乳腺癌抑制候选基因-1（BCSC-1）是一种最近被发现的候选肿瘤抑制基因。在先前的研究中，已在多种癌症类型中检测到BCSC-1水平降低。基质金属蛋白酶（MMP）-14是一种膜型MMP，在肿瘤进展和预后中起重要作用。先前的研究表明，MMP-14在不同癌症类型中高表达，并通过重塑细胞外基质促进肿瘤侵袭或转移。然而，近年来关于BCSC-1和MMP-14在人类乳腺癌中的报道较少。在本研究中，调查了BCSC-1和MMP-14与人类乳腺癌的关联。免疫组织化学分析结果显示，与相邻正常乳腺组织相比，乳腺癌组织中BCSC-1表达降低，MMP-14表达上调。定量聚合酶链反应和蛋白质印迹分析也表明，与健康乳腺组织相比，乳腺癌组织中BCSC-1表达水平显著降低，MMP-14表达水平显著升高。此外，BCSC-1表达降低和MMP-14表达上调与肿瘤细胞分化、淋巴结转移和远处转移有关。还对BCSC-1和MMP-14进行了相关性分析，结果表明两者呈负相关。总之，目前的研究结果表明，在人类乳腺癌中BCSC-1表达下调，而MMP-14表达上调。这两个基因可能在人类乳腺癌发展过程中起重要作用。
