Wang Zhiwei, Zhang Jie, Fan Guangpu, Zhao Hui, Wang Xu, Zhang Jing, Zhang Peide, Wang Wei
Cardiovascular Surgery Department, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, 167 Beilishi Road, Beijing, 100037, China.
Department of Cardiovascular Surgery, Peking University People's Hospital, 11 Xizhimen South Road, Beijing, 100044, China.
Biomed Opt Express. 2018 Jan 4;9(2):423-436. doi: 10.1364/BOE.9.000423. eCollection 2018 Feb 1.
We developed a new method, SUT (Scheme Update on tissue Transparency), to view cardiac microstructures and unveil the molecular changes underlying cardiac diseases. SUT is an effective method to clear whole-hearts from different species. Over the course of 4 - 6 days we obtained transparent whole-layer left ventricular tissues from mice with only an approximate 1% protein loss. In addition, EAL (Electrophoretic Antibody Labeling) was used to achieve fast antibody labeling by electric force, which significantly reduced antibody incubation time from days to hours. SUT, together with EAL and modern imaging techniques, were successfully used to visualize three-dimensional spatial distribution of various molecules in cardiac tissue. We also observed changes in the number and phenotypes of fibroblasts during post-myocardial infarction in a stereoscopic pattern. We believe that our technique opens a new avenue to explore the mechanisms underlying cardiac diseases.
我们开发了一种新方法——组织透明化方案更新（SUT），用于观察心脏微观结构并揭示心脏病背后的分子变化。SUT是一种清除不同物种全心脏组织的有效方法。在4至6天的时间里，我们从小鼠身上获得了透明的全层左心室组织，蛋白质损失仅约1%。此外，电泳抗体标记（EAL）通过电力实现了快速抗体标记，显著将抗体孵育时间从数天缩短至数小时。SUT与EAL以及现代成像技术一起，成功用于可视化心脏组织中各种分子的三维空间分布。我们还以立体模式观察了心肌梗死后成纤维细胞数量和表型的变化。我们相信我们的技术为探索心脏病的发病机制开辟了一条新途径。
