Ginner Laurin, Schmoll Tilman, Kumar Abhishek, Salas Matthias, Pricoupenko Nastassia, Wurster Lara M, Leitgeb Rainer A
Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Austria.
Christian Doppler Laboratory for Innovative Optical Imaging and its Translation to Medicine, Medical University of Vienna, Austria.
Biomed Opt Express. 2018 Jan 8;9(2):472-485. doi: 10.1364/BOE.9.000472. eCollection 2018 Feb 1.
We demonstrate a high-resolution line field en-face time domain optical coherence tomography (OCT) system using an off-axis holography configuration. Line field en-face OCT produces high speed en-face images at rates of up to 100 Hz. The high frame rate favors good phase stability across the lateral field-of-view which is indispensable for digital adaptive optics (DAO). Human retinal structures are acquired in-vivo with a broadband light source at 840 nm, and line rates of 10 kHz to 100 kHz. Structures of different retinal layers, such as photoreceptors, capillaries, and nerve fibers are visualized with high resolution of 2.8 µm and 5.5 µm in lateral directions. Subaperture based DAO is successfully applied to increase the visibility of cone-photoreceptors and nerve fibers. Furthermore, en-face Doppler OCT maps are generated based on calculating the differential phase shifts between recorded lines.
我们展示了一种使用离轴全息配置的高分辨率线场正面时域光学相干断层扫描（OCT）系统。线场正面OCT以高达100 Hz的速率产生高速正面图像。高帧率有利于在横向视野范围内实现良好的相位稳定性，这对于数字自适应光学（DAO）是必不可少的。使用840 nm的宽带光源在体内获取人类视网膜结构，线速率为10 kHz至100 kHz。不同视网膜层的结构，如光感受器、毛细血管和神经纤维，在横向方向上以2.8 µm和5.5 µm的高分辨率可视化。基于子孔径的DAO成功应用于提高视锥光感受器和神经纤维的可见性。此外，基于计算记录线之间的差分相移生成正面多普勒OCT图。
