Rosenberry Ryan, Chung Susie, Nelson Michael D
Applied Physiology and Advanced Imaging Laboratory, Department of Kinesiology, University of Texas at Arlington.
Applied Physiology and Advanced Imaging Laboratory, Department of Kinesiology, University of Texas at Arlington;
J Vis Exp. 2018 Feb 20(132):57317. doi: 10.3791/57317.
Exercise represents a major hemodynamic stress that demands a highly coordinated neurovascular response in order to match oxygen delivery to metabolic demand. Reactive hyperemia (in response to a brief period of tissue ischemia) is an independent predictor of cardiovascular events and provides important insight into vascular health and vasodilatory capacity. Skeletal muscle oxidative capacity is equally important in health and disease, as it determines the energy supply for myocellular processes. Here, we describe a simple, non-invasive approach using near-infrared spectroscopy to assess each of these major clinical endpoints (reactive hyperemia, neurovascular coupling, and muscle oxidative capacity) during a single clinic or laboratory visit. Unlike Doppler ultrasound, magnetic resonance images/spectroscopy, or invasive catheter-based flow measurements or muscle biopsies, our approach is less operator-dependent, low-cost, and completely non-invasive. Representative data from our lab taken together with summary data from previously published literature illustrate the utility of each of these end-points. Once this technique is mastered, application to clinical populations will provide important mechanistic insight into exercise intolerance and cardiovascular dysfunction.
运动是一种主要的血流动力学应激，需要高度协调的神经血管反应，以便使氧气输送与代谢需求相匹配。反应性充血（对短暂的组织缺血作出反应）是心血管事件的独立预测指标，并为血管健康和血管舒张能力提供重要见解。骨骼肌氧化能力在健康和疾病中同样重要，因为它决定了肌细胞过程的能量供应。在此，我们描述了一种简单的非侵入性方法，使用近红外光谱在单次门诊或实验室就诊期间评估这些主要临床终点（反应性充血、神经血管耦合和肌肉氧化能力）中的每一项。与多普勒超声、磁共振成像/光谱、基于侵入性导管的流量测量或肌肉活检不同，我们的方法对操作人员的依赖性较小、成本低且完全非侵入性。我们实验室的代表性数据与先前发表文献的汇总数据一起说明了这些终点中每一项的效用。一旦掌握了这项技术，将其应用于临床人群将为运动不耐受和心血管功能障碍提供重要的机制性见解。
