Lutz C T, Hollifield W C, Seed B, Davie J M, Huang H V
Proc Natl Acad Sci U S A. 1987 Jul;84(13):4379-83. doi: 10.1073/pnas.84.13.4379.
The Syrinx 2A phage and pi AN13 plasmid were designed for screening of DNA libraries by homologous recombination in vivo. Syrinx 2A carries multiple cloning sites and a recently identified lambda gene, rap (recombination adept with plasmid), required for efficient phage-plasmid recombination. We describe a rapid, reliable, and technically easy method to screen Syrinx 2A libraries, expand the resulting phage-plasmid cointegrates, and subclone plasmid in as little as 2 days. Recombination screening allows one specific member of a closely related multigene family to be isolated selectively.
Syrinx 2A噬菌体和pi AN13质粒设计用于通过体内同源重组筛选DNA文库。Syrinx 2A携带多克隆位点和一个最近鉴定出的λ基因rap（与质粒重组能力强），这是高效噬菌体 - 质粒重组所必需的。我们描述了一种快速、可靠且技术上简便的方法，可在短短2天内筛选Syrinx 2A文库、扩增产生的噬菌体 - 质粒共整合体并亚克隆质粒。重组筛选能够选择性地分离密切相关的多基因家族中的一个特定成员。
