Zhao Yuanyuan, Wang Zhanyong, Zhang Jing, Su Tingting
College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun, 113001 China.
3 Biotech. 2018 Mar;8(3):181. doi: 10.1007/s13205-018-1198-y. Epub 2018 Mar 14.
The production of Hasma generates plentiful non-edible by-products in China and Central Asia. As one of main by-products, the skin of is discarded as waste without utilization. In this work, skin collagen (RCSC) hydrolysates were extracted using pepsin under acidic conditions. The yield of RCSC is 15.1% (w/w). Amino acid analysis revealed that RCSC contained glycine (204.5/1000 residues) and imino acids (182/1000 residues). RCSC exhibited high solubility in acidic pH (1-4) and low NaCl concentrations (< 2%, w/v). Differential scanning calorimetry indicated that the denaturation temperature of RCSC was 33.5 °C. Scanning electron microscopy analysis confirmed their well-defined fibril morphologies. The results indicated that the skin of is an alternative source of collagen hydrolysates, and RCSC can serve as a potential source applying in foodstuff and medical industry.
在中国和中亚地区，哈士蟆的生产会产生大量不可食用的副产品。作为主要副产品之一，哈士蟆皮被当作废弃物丢弃而未加以利用。在本研究中，采用胃蛋白酶在酸性条件下提取哈士蟆皮胶原蛋白（RCSC）水解物。RCSC的得率为15.1%（w/w）。氨基酸分析表明，RCSC含有甘氨酸（204.5/1000个残基）和亚氨基酸（182/1000个残基）。RCSC在酸性pH值（1 - 4）和低NaCl浓度（<2%，w/v）下具有高溶解性。差示扫描量热法表明，RCSC的变性温度为33.5℃。扫描电子显微镜分析证实了其明确的原纤维形态。结果表明，哈士蟆皮是胶原蛋白水解物的替代来源，RCSC可作为一种潜在来源应用于食品和医药工业。
