Su Feng, Chen Xin, Liu Xin, Liu Guanghui, Zhang Yong
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, Shandong, China.
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
AMB Express. 2018 Mar 20;8(1):42. doi: 10.1186/s13568-018-0573-8.
Bovine tuberculosis is a disease caused by Mycobacterium bovis (M. bovis) that leads to great economic losses in cattle production. The discovery of a reasonable bioagent to reduce M. bovis infection risk and environment contamination becomes significant and urgent. Previous study reported that human β-defensin-3 (HBD3) participated in Mycobacterial immunity and was recognized as a suitable candidate reagent. However, its minimal inhibitory concentration to M. bovis is not yet reported. In this study, we first purified HBD3 protein by recombinant-DNA technology and prokaryotic expression system. Subsequently, anti-bacterial tests were used to evaluate the basic bioactivity of the protein. Results revealed that recombinant HBD3 (rHBD3) protein inhibits Staphylococcus multiplication but not the host Escherichia coli. The growth curve of M. bovis showed that rHBD3 protein controls the proliferation of M. bovis in 20 μg/ml concentration. In addition, rHBD3 protein-incubated M. bovis exhibited reduced infectivity to alveolar epithelial cells and macrophages. In conclusion, the expression of rHBD3 protein is a potential ideal bio-regent for reducing M. bovis infection.
牛结核病是一种由牛分枝杆菌引起的疾病，会给养牛业造成巨大经济损失。发现一种合理的生物制剂以降低牛分枝杆菌感染风险和环境污染变得至关重要且迫在眉睫。先前的研究报道，人β-防御素3（HBD3）参与了分枝杆菌免疫，被认为是一种合适的候选试剂。然而，其对牛分枝杆菌的最小抑菌浓度尚未见报道。在本研究中，我们首先通过重组DNA技术和原核表达系统纯化了HBD3蛋白。随后，采用抗菌试验评估该蛋白的基本生物活性。结果显示，重组HBD3（rHBD3）蛋白可抑制金黄色葡萄球菌的增殖，但对宿主大肠杆菌无抑制作用。牛分枝杆菌的生长曲线表明，rHBD3蛋白在20μg/ml浓度下可控制牛分枝杆菌的增殖。此外，用rHBD3蛋白孵育的牛分枝杆菌对肺泡上皮细胞和巨噬细胞的感染性降低。总之，rHBD3蛋白的表达是一种潜在的理想生物试剂，可用于降低牛分枝杆菌感染。
