Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, Canada.
Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
Front Neural Circuits. 2018 Mar 6;12:17. doi: 10.3389/fncir.2018.00017. eCollection 2018.
Considerable research effort has been invested into the transplantation of mammalian photoreceptors into healthy and degenerating mouse eyes. Several platforms of rod and cone fluorescent reporting have been central to refining the isolation, purification and transplantation of photoreceptors. The tracking of engrafted cells, including identifying the position, morphology and degree of donor cell integration post-transplant is highly dependent on the use of fluorescent protein reporters. Improvements in imaging and analysis of transplant recipients have revealed that donor cell fluorescent reporters can transfer into host tissue though a process termed material exchange (ME). This recent discovery has chaperoned a new era of interpretation when reviewing the field's use of dissociated donor cell preparations, and has prompted scientists to re-examine how we use and interpret the information derived from fluorescence-based tracking tools. In this review, we describe the status of our understanding of ME in photoreceptor transplantation. In addition, we discuss the impact of this discovery on several aspects of historical rod and cone transplantation data, and provide insight into future standards and approaches to advance the field of cell engraftment.
大量的研究工作投入到了哺乳动物光感受器向健康和退化的小鼠眼睛中的移植。几种棒状和锥状荧光报告平台对于改进光感受器的分离、纯化和移植至关重要。移植细胞的跟踪，包括确定移植后供体细胞的位置、形态和整合程度，高度依赖于荧光蛋白报告基因的使用。对移植受者的成像和分析的改进表明，供体细胞荧光报告基因可以通过一种称为物质交换（ME）的过程转移到宿主组织中。这一最新发现开创了一个新的时代，使人们对该领域使用分离的供体细胞制剂进行了重新解读，并促使科学家重新审视我们如何使用和解释基于荧光的跟踪工具得出的信息。在这篇综述中，我们描述了我们对光感受器移植中 ME 的理解现状。此外，我们还讨论了这一发现对历史上棒状和锥状移植数据的几个方面的影响，并为推进细胞移植领域的未来标准和方法提供了一些见解。
