Department of General Surgery, Shanghai Traditional Chinese Medicine-Integrated Hospital, Shanghai, P.R. China.
Oncol Res. 2019 Mar 29;27(4):439-447. doi: 10.3727/096504018X15214994641786. Epub 2018 Mar 21.
Tripartite motif-containing 14 (TRIM14) is abnormally expressed in several human cancers. However, the function and expression of TRIM14 in human breast cancer are still largely unknown. To understand the biological function of TRIM14 in breast cancer, we measured the expression level of TRIM14. Cell proliferation and cell apoptosis were measured after TRIM14 overexpression or knockdown. Upregulation of TRIM14 was found in human breast cancer specimens and cell lines. Reduction of TRIM14 inhibited cell proliferation but increased cell apoptosis in the BT474 and MDA-MB-231 cell lines. Further study showed that knockdown of TRIM14 upregulated the expression of BAX while downregulating the expression of BCL2. In addition, the expression of SHP-1 was increased, and the phosphorylation of STAT3 (p-STAT3) was inhibited. Conversely, overexpression of TRIM14 had the opposite effects. Additionally, cryptotanshinone, a STAT3 inhibitor, inhibited cell proliferation but increased cell apoptosis in the BT474 and MDA-MB-231 cell lines. In conclusion, TRIM14 may act as an oncogene in human breast cancer and may be a novel strategy for human breast cancer.
三结构域蛋白 14（TRIM14）在几种人类癌症中异常表达。然而，TRIM14 在人类乳腺癌中的功能和表达仍知之甚少。为了了解 TRIM14 在乳腺癌中的生物学功能，我们测量了 TRIM14 的表达水平。在过表达或敲低 TRIM14 后，测量细胞增殖和细胞凋亡。在人乳腺癌标本和细胞系中发现 TRIM14 上调。降低 TRIM14 的表达抑制了 BT474 和 MDA-MB-231 细胞系中的细胞增殖，但增加了细胞凋亡。进一步的研究表明，敲低 TRIM14 上调了 BAX 的表达，同时下调了 BCL2 的表达。此外，SHP-1 的表达增加，STAT3（p-STAT3）的磷酸化受到抑制。相反，过表达 TRIM14 则产生相反的效果。此外，STAT3 抑制剂 cryptotanshinone 抑制了 BT474 和 MDA-MB-231 细胞系中的细胞增殖，但增加了细胞凋亡。总之，TRIM14 可能在人类乳腺癌中充当癌基因，并且可能成为人类乳腺癌的一种新策略。
