使用双光子激光扫描显微镜对胎儿腹腔内器官进行活体成像。

Live Imaging of Fetal Intra-abdominal Organs Using Two-Photon Laser-Scanning Microscopy.

作者信息

Koike Yuhki, Li Bo, Chen Yong, Miyake Hiromu, Lee Carol, Chi Lijun, Wu Richard, Inoue Mikihiro, Uchida Keiichi, Kusunoki Masato, Delgado-Olguin Paul, Pierro Agostino

机构信息

Translational Medicine Program, Division of General and Thoracic Surgery, The Hospital for Sick Children, Toronto, ON, Canada.

Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, Tsu, Mie, Japan.

出版信息

Methods Mol Biol. 2018;1752:63-69. doi: 10.1007/978-1-4939-7714-7_6.

DOI:10.1007/978-1-4939-7714-7_6

PMID:29564762

Abstract

The processes by which the intra-abdominal organ circulatory system develops in the embryo and during organogenesis are unclear. Previous studies have used fixed tissues to study the development of abdominal organ vasculature in the embryo; however, the intravital circulation of intra-abdominal organs in rodent fetal development has not been studied. This protocol describes a system that uses two-photon laser-scanning microscopy (TPLSM) for real-time observation and quantification of normal and pathologic live fetal intra-abdominal dynamics while the fetus is still connected to the mother via the umbilical cord.

摘要

胚胎期及器官发生过程中腹腔内器官循环系统的发育过程尚不清楚。以往的研究使用固定组织来研究胚胎腹部器官脉管系统的发育；然而，啮齿类动物胎儿发育过程中腹腔内器官的活体循环尚未得到研究。本方案描述了一种系统，该系统使用双光子激光扫描显微镜（TPLSM）对正常和病理状态下的胎儿腹腔内实时动态进行观察和定量分析，此时胎儿仍通过脐带与母体相连。