Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Research and Development Center of TCM, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China.
J Food Drug Anal. 2018 Apr;26(2):807-814. doi: 10.1016/j.jfda.2017.10.009. Epub 2017 Dec 1.
Fructus Psoraleae, a traditional Chinese medicine, is widely used for preventing and treating various diseases such as vitiligo, osteoporosis and psoriasis. Coumarin, such as psoralenoside, isopsoralenoside, psoralen and isopsoralen, are important compounds in Fructus Psoraleae. In our study, ultra performance liquid chromatography coupled with diode array detector was employed for an excellent method validation for simultaneous quantification of psoralenoside, isopsoralenoside, psoralen and isopsoralen, which was further applied in performing general survey of Fructus Psoraleae from the different origins and chemical identification of the roasted from raw Fructus Psoraleae in the light of illuminating the transformed rule of psoralenoside and isopsoralenoside. There is a reciprocal relationship between (iso)psoralenoside and (iso)psoralen, and the total content remains balance in Fructus Psoraleae from the different origins. In addition, we found that (iso)psoralenoside in the powder of the raw Fructus Psoraleae could be easily transformed into (iso)psoralen in methanol aqueous solution, especially above 50% water, rather than the roasted one. Thus, we proposed a hypothesis that transformation between (iso)psoralenoside and (iso)psoralen was hindered by inactivation of β-glucosidase in the process of roasting Fructus Psoraleae, which was further verified by observing transformation of (iso)psoralenoside under the different conditions, such as temperature, pH and β-glucosidase. Therefore, we developed a feasible method to distinguish the roasted from raw Fructus Psoraleae by observing conversion from (iso)psoralenoside to (iso)psoralen in 50% methanol aqueous solution. In summary, these results pave the way for elevating quality standard for Fructus Psoraleae and distinguishing the salt-processed from raw Fructus Psoraleae.
补骨脂，一种传统的中药，被广泛用于预防和治疗各种疾病，如白癜风、骨质疏松症和银屑病。香豆素，如补骨脂素、异补骨脂素、补骨脂素和异补骨脂素，是补骨脂中的重要化合物。在我们的研究中，采用超高效液相色谱-二极管阵列检测器（UPLC-DAD）建立了一种同时定量分析补骨脂素、异补骨脂素、补骨脂素和异补骨脂素的方法，并进一步应用于不同来源的补骨脂总香豆素的普查，以及对生品和盐炙品的化学鉴别，以阐明补骨脂素和异补骨脂素的转化规律。（异）补骨脂素和（异）补骨脂素之间存在相互转化的关系，不同来源的补骨脂素总含量保持平衡。此外，我们发现生品补骨脂粉在甲醇水溶液中，尤其是在 50%以上的水相中，（异）补骨脂素很容易转化为（异）补骨脂素，而盐炙品则不然。因此，我们提出了一个假设，即在炮制补骨脂的过程中，β-葡萄糖苷酶失活，阻碍了（异）补骨脂素和（异）补骨脂素之间的转化，这一假设通过观察不同条件下（如温度、pH 值和β-葡萄糖苷酶）（异）补骨脂素的转化得到了进一步验证。因此，我们通过观察 50%甲醇水溶液中（异）补骨脂素向（异）补骨脂素的转化，建立了一种区分生品和盐炙品的可行方法。总之，这些结果为提高补骨脂的质量标准和区分盐制品和生品奠定了基础。
