Wang Dan-Ni, Guo Jia-Ming, Chai Xin, Yang Jing, Wang Yue-Fei
Tianjin Key Laboratory of TCM Chemistry and Analysis, State Key Laboratory of Component-based Chinese Medicine,Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine Tianjin 301617, China.
Zhongguo Zhong Yao Za Zhi. 2022 Aug;47(15):4089-4097. doi: 10.19540/j.cnki.cjcmm.20220421.202.
The present study established a determination method of Psoraleae Fructus by quantitative analysis of multi-components by the single marker(QAMS) and further improved the thin-layer chromatography(TLC) method. The QAMS method was established by UPLC with psoralen as the internal marker, and the content of psoralenoside, isopsoralenoside, psoralen, and isopsoralen was simultaneously determined. As revealed by the comparison with results of the external standard method, the QAMS method was accurate and feasible. According to the current quality standards of Psoraleae Fructus, the TLC method was further optimized and improved, and bakuchiol was added for identification based on the original TLC method with psoralen and isopsoralen as indicators. This study provides a reference for improving the quality control method of Psoraleae Fructus.
本研究建立了基于单指标多成分定量法（QAMS）的补骨脂测定方法，并进一步改进了薄层色谱（TLC）法。以补骨脂素为内标，采用超高效液相色谱法建立QAMS法，同时测定补骨脂苷、异补骨脂苷、补骨脂素和异补骨脂素的含量。与外标法结果比较表明，QAMS法准确可行。根据补骨脂现行质量标准，在以补骨脂素和异补骨脂素为指标的原TLC法基础上，进一步优化改进TLC法，增加了对毛蕊异黄酮的鉴别。本研究为完善补骨脂质量控制方法提供了参考。
